| Juanbilijieqingfang can be used to treat and alleviate gout and acute gouty arthritis,The clinical practice and animal experiments show that it has a good anti-inflammatory and analgesic effect.This experiment is designed according to the idea of this functions and indications?In this analgesic experiment,we studies its analgestic and anti-inflammatory effects with pain animales models and acute gouty arthritis animales model.In this study,using juanbilijieqingfang as the main observation,the relevant inflammatory factors as the entry point,the acute gouty arthritis model of drug intervention,in-depth study the mechanism of juanbilijiqingfang its treatment on gouty arthritis;so as to provide a scientific basis for clinical application and experimental evidence.Objietive:1.The analgesic effect of juanblijieqingfang was studied by different analgesia experiments that reveals the mechanism of analgesic effect.2.to explory the regulation effect of juanbilijieqingfang to the gout arthritis and to study its mechanism of anti-gouty arthritis.Methods:1.Analgesic effects of juanbilijieqingfang were observed on two pain modeles,such as pain model induced by hot plate,writhing response induces by acetic acid test in rats.In this study,the rats were given gavage of low,medium and high dose of juanbiliqiqingfang solution to observe the changes of pain threshold in rarts in experiment.,the experiment that writhing response induces by acetic acid test in rats,we observed the number of writhing in rats and the rate of writhing inhibition,futher more,we measured the levels of prostaglandin E2?PGE2?and nitric oxide?NO?in serum in rats and to compare the analgesic effect of juanbilijieqingfang decoction and explore its mechanism of analgesic effect by comparing with diclofenac sodium,a positive control drug.2.Intervention of acute gouty arthritis model:60 rats were divided into six groups from A to F with each group 10 rats.Group A represents normal group.B is Model control group,C,D,E on behalf of juanbilijieqingfang high,medium and low dose group,F represents the positive drug etoposide treatment group;then,establishing the rat model of gouty arthritis in the knee joint,using the juanbilijiqingfang intervened the rats,compared by etoposide as a positive control drug.All the rats were interfered for 48 hours,and we Observed the knee knee inflammation index,measured the knee circumference of the rats and calculated the swelling of the knee joint 4h,12h,24h and 48h after modeling,After the experiment,the levels of IL-1?,IL-6 and TNF-?in synovial fluid of knee joint were measured by enzyme-linked immunosorbent assay?ELISA?.Results:1.In Analgesic effects experiments:The amount of writhing was increased and Inhibit the wrinkle rate of rats in each dose groups of juanbilijiqingfang,there was significant difference compared with the blank control group,There is a dose-effect relationship in in each dose groups of juanbilijiqingfang.The levels of serum PGE2and NO were decreased in each dose group,and the analgesic effect was obvious in high dose group compared with Low,middle and high three dose groups.2.The dosage of each dose group could prolong the latency of licking foot in rats and increase the pain threshold of rats,which was higher than that of blank control group.After 90min and 120min,the difference between high and low dose group was obvious.With the increase of drug dose,Pain effect is obvious,there is a certain dose-effect relationship.Conelusion:1.In the Analgesia experiment,rats can be extended in the hot plat method of licking the foot latency,and improve the hot plate method in rat pain threshold level in in each dose groups of juanbilijiqingfang,The above shows that juanbilijieqingfang has a good analgesic effects,and its mechanism may inhibit the expression of inflammatory factors PGE2 and NO,and there is dose-effect relationship between traditional Chinese medicine compound.2.Juanbilijieqingfang can effectively reduce the experimental gouty arthritis model of arthritis index,decreasing the level of erythrocyte count in synovial fluid of experimental gouty arthritis rats and the effect of TNF-?,IL-6 and IL-1? on the expression in the synovial fluid of knee joint Of the content,thereby inhibiting the inflammatory response.This study provides experimental basis for the treatment of gouty arthritis by the method of clearing heat and dampness,and also provides important sound scientific evidence and research route for the development of treating gouty arthritis. |
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