Effect Of Corn Oil-Derived Sterols On Regulating Cholesterol Cholesterol Metalolism And Intestinal Microbiota In High Fat-High Cholesterol Diet Fed Hamsters

Phytosterols are natural active compounds in plants and widespread in vegetable(plant)oils,nuts and seeds.The chemical structures of phytosterols are similar to cholesterol.Phytosterol functions as a structural component in plant membranes and regulates cell proliferation and differentiation.The efficacy of phytosterols in cholesterol-lowering,anti-inflammatory and antioxidation has been known.The aim of this report was to investigate the effects of corn oil-derived sterols on cholesterol-lowering,cholesterol regulation and intestinal microbiota in Golden Syrian hamsters fed high fat-high cholesterol diet,providing a basis for further research in effects of corn oil-derived sterols on metabolism by intestinal microbiota and has guiding significance to develop related products.Totally 45 hamsters were randomly divided into normal group,high fat-high cholesterol(HFHC)group and three experimental groups with 1,5 and 10 g/kg of corn oil-derived sterols.Serum TC,TG and HDL-C were detected.The content of hepatic cholesterol,fecal neutral and acidic sterols was determined by GC.The expression of cholesterol-regulating genes in the liver and small intestine was assayed with qPCR.The changes of intestinal microbiota were assayed by DGGE.The results of blood lipid determination shown that 50‰ and 10‰ of corn oil-derived sterols significantly decreased TC,TG,non HDL-C and non HDL-C/HDL-C(P<0.05).The results of qPCR shown that the RNA expression level in liver of LXR?,HMG-CoA-R and LDL-R was significantly increased in 5‰ and 10‰ group(P<0.01),and CYP7A1 was significantly increased in 10‰ group(P<0.01).The RNA expression level in small intestine of HMG-CoA-R,ABCG5,ABCG8 and MTP was significantly increased in 5‰and 10‰ group.The content of hepatic cholesterol was significantly decreased in 5‰ and 10‰ group.The content of fecal neutral and acidic sterols-in fecal was increased with increasing dose of corn oil-derived sterol.The results of DGGE shown that the diversity was increased induced by high fat high cholesterol diet.Oscillibacter sp.,Lactobacillus sp.,Desulfovibrio sp.are significantly increased and Akkermansia sp.,butyrate-producting Anaerostipes sp.are significantly decreased(P<0.05).The diversity and bacteria was restored to a certian extent.It It was concluded that corn oil-derived sterols improved intestinal flora disturbance induced by high fat high cholesterol diet.In conclusion,cholesterol-lowering mechanism of corn oil-derived sterol probably was in increasing cholesterol excretion,inhibiting cholesterol absorption and synthesis,improving intestinal microbiota.