Study On Quality Standard Of The Root Of Lycium Chinense Mill.

ObjectiveAs the basis of Chinese Pharmacopoeia(2010),establish the quality evaluation method of the root of Lycium chinense Mill.,and set up a quality standard of it.What we have done is for the root of Lycium chinense Mill.to he collected by standards of Guangdong province,and it will help to guide the safety and effectiveness in clinic.MethodsQuality standard of the root of Lycium chinense Mill.was established according to Chinese Pharmacopoeia(2010),concrete research content including microscopic identification,thin layer chromatography(TLC),water content,total ashes,acid-insoluble ashes,and extractives and extractives.Measure five kinds of heavy metal content including copper,lead,cadmium,arsenic,mercury and the total amount of heavy metals.Establish the determination of organochlorine pesticide residues in the root of Lycium chinense Mill..Establish HPLC of ferulic acid and chlorogenic acid in the root of Lycium chinense Mill..Establish HPLC of betaine in the root of Lycium chinense Mil..Results1?Resource distribution of the root of Lycium chinense Mill.The root of Lycium chinense Mill.in all parts of our country have distribution,produce of it in guangdong province is mainly distributed in zengcheng,panyu,foshan,dongguan and other cities and counties.2?Germplasm identification of the root of Lycium chinense Mill.The first is through in-depth panyu,kaiping,guangdong zhaoqing etc region,confirm the documents in the form of the original plant to its description.The second is through books recorded in combination with the collected samples,determine its medicinal traits.Third is by observing the microscopic characteristics of the root of Lycium chinense Mill.,the outer cortex and cork layer,phloem,xylem and its characteristic of sand crystal,the description of the fine characteristics of starch granule etc.The fourth is to establish a TLC method of the root of Lycium chinense Mill..Through the optimization of the extraction conditions,an optimization system,color,and examine the way of optimizing finally established is suitable for thin layer chromatographic conditions.3?Analysis and evaluation of the root of Lycium chinense Mil.Determine moisture,total ash,acid insoluble ash content,water soluble extract,alcohol soluble extract.Provide a reference for the establishment of the root of Lycium chinense Mill.medicinal quality standard.4?The safety of the root of Lycium chinense Mill.Mainly the determination of copper,lead,mercury,cadmium,arsenic,five kinds of heavy metal content,and through the total amount of heavy metals is determined by colorimetric method.Results conform to the "green trade standards of importing medicinal plants and the preparations" requirements.Also in the determination of the root of Lycium chinense Mill.eight kinds of organochlorine pesticide residues.Results are not checked out the eight ingredients,with the root of Lycium chinense Mill.grows mainly in guangdong is wild about.5?Effective composition content of the root of Lycium chinense Mill.In the root of Lycium chinense Mill.were determined and analyzed by using the method of HPLC of chlorogenic acid,ferulic acid,betaine.To fill the blank of the root of Lycium chinense Mill.medicinal material content determination of provide reference basis.ConclusionEstablish a quality standard of the root of Lycium chinense Mill.,for quality control of it with modern technology and methods of qualitative and quantitative analysis and evaluation,also for the root of Lycium chinense Mill.to be collected by the standards of Guangdong province,and to guide the safety and effectiveness in clinic.