"Benxu" And "Biaoshi" Of Colorectal Cancer Intestinal Tissue In Different Parts Of CAF Differential Expression Of CAF Related Markers In Intestinal Tissues

Background:With the development of traditional Chinese medicine(TCM),the intervention of traditional Chinese medicine(TCM)has many advantages,such as multiple links,multiple approaches and multiple targets.Systemic or local blood stasis,phlegm,dampness,spleen and kidney is good soil and environment--"cancer drug diffusion and metastasis of missed and not go to the area of interest from it".In this study,analysis of intestinal tissue in different parts of "standard" and "deficiency" syndrome of tumor associated fibroblast marker expression,explore the CAF related markers in colorectal carcinogenesis.The expression and characteristics of TCM syndromes and CAFs mechanism in tumor development has no correlation.Specific:Study on spleen deficiency,blood of two deficiency mainly "deficiency" syndrome and damp heat,blood stasis and toxin resistance,stagnation of liver qi "standard" card of two kinds of syndromes in patients with colorectal cancer tissues,cancer tissues and cancer related markers expression difference analysis,and based on two card type,tumor tissues were observed in patients with colorectal cancer associated fibroblasts after alpha-SMA,FAP,S100A6 and PDPN,the expression of SPARC five protein markers,and understand the relationship between the expression of these 5 proteins and colorectal cancer,in order to reveal the tumor associated fibroblast markers in the "virtual" and "real,two kinds of syndromes of colorectal cancer development may function and its clinical significance.Method:The experimental targets for hospitalized patients in First Affiliated Hospital of Guangzhou University of Chinese Medicine anorectal April 2015-2016 year in September period,early admission first syndrome questionnaire,collecting clinical data.and other required information.After surgery and pathology showed colorectal cancer patients in the hospital,such patients in line with the survey,do not belong to the criteria for colorectal cancer removed.At the end of this period,all eligible patients were prospectively investigated for clinical syndrome.To observe the general epidemiological data and clinical symptoms,signs,tongue and pulse,making clinical questionnaire,and engaged in clinical work for more than 3 years of senior TCM doctors to observe,initially identified colorectal cancer syndrome types.In accordance with the relevant norms of TCM "diagnosis of anorectal common disease diagnosis and treatment guidelines" in 1,and Dukes AJCC/UICC stage,TNM stage(2002)colorectal cancer staging criteria accurately.The relevant diagnostic criteria does not conform to,such as have cardiovascular disease,liver and kidney damage and other hematopoietic system disease,mental disorders affect the TCM syndrome description,judgment patients,and finally incomplete information also needs to be removed.There was no history of chemotherapy before surgery,and the pathological results after surgery for colorectal cancer patients included in the scope of the study.In accordance with the above criteria.to select cases,fill in clinical research observation table,and the data into the database,induction,statistical analysis.Among them,there were 23 males(51.1%)and female(48.9%)().The average age of the patients was 41-82 years old,and the age of the patients was 62.2 years old.The main pathological types were adenocarcinoma of the middle differentiation,lymph node metastasis in 15 cases.and no metastasis in all cases.Clinical analysis and descriptive analysis of clinical symptoms of tongue and pulse analysis using sample cluster analysis methods for 67 patients,statistical analysis,preliminary draw syndrome type colorectal cancer early and middle disease syndrome diagnosis,the main clinical symptoms.The four diagnostic information frequency>15%of the symptoms and signs of 26,respectively,fatigue,God tired lazy body,difficulty sleeping,dizziness,poor appetite,hi cold,thirst without desire to drink,mouth parched and tongue scorched,halitosis,pharyngeal obstruction,koudan mouth,sticky mouth or clear saliva,stuffy chest vomiting and abdominal distension pain,abdominal distension,Yaoxisuanruan,tenesmus,anal bulge,will not do,constipation and melena,loose stool,mucous stool,cold chills,limbs cold,irritability,sweating.The abdominal pain,abdominal distension,endless,mucous bloody stool,tenesmus,anal bulge for new onset of symptoms in the most common.The frequency of more than 15%tongue 4,respectively,red tongue,greasy fur,yellow fur,white fur,thick fur.The frequency of more than 15%pulse 6,respectively,and the pulse string pulse,soft pulse and rapid pulse,deep pulse,thready pulse.Result:The above positive symptoms of cluster analysis,the preliminary conclusion syndromes following 4 syndromes:blood stasis and toxin in stasis,spleen deficiency,deficiency of Qi and blood two,rdhs.The blood stasis poison inside and damp heat accumulation as "standard",the remaining 2 cards are classified as "the virtual card".Blood stasis poison inside symptoms:abdominal pain,bloody stool,dark purple,tenesmus,difficult defecation,dark red tongue,pulse string corrosion;the main symptoms:abdominal pain,rdhs dysenteric diarrhea,tenesmus,chest tightness,nausea and anorexia,red tongue with yellow greasy fur,slippery pulse number;the main symptoms of spleen deficiency syndrome:pale chlorosis,Yaoxisua.nruan,chills,abdominal pain,diarrhea due to the increase in the number of pale tongue,thin white fur,thready pulse weakness;deficiency of Qi and blood deficiency main symptoms:thin,pale,shortness of breath,abdominal distension,diarrhea,constipation,pale tongue,thin white fur,thin and weak pulse.Immunohistochemical test and PCR test "standard" and "deficiency"syndrome of colorectal cancer intestinal tissue in different parts of CAF markers,the results showed that tumor associated fibroblast marker alpha-SMA,FAP,S100A6 and PDPN,the expression of SPARC in colorectal cancer tissues showing obvious difference with normal tissue and cancer.Paracancerous tissues were not significantly different.However,there were no significant differences between the two groups in terms of the expression of the 5 proteins in the same group.Conclusion:1、referring to the relevant diagnostic criteria,the development of colorectal cancer clinical syndrome observation table,into the patients with differentiated adenocarcinoma.2、using cluster analysis method,the standard is divided into blood stasis poison inside syndrome,spleen deficiency syndrome,deficiency of Qi and blood two,rdhs.The blood stasis poison inside and damp heat accumulation as "standard",the remaining 2 cards are classified as "the virtual card"..3、the tumor associated fibroblast marker alpha-SMA,FAP,S100A6 and PDPN,there was significant difference of SPARC expression in different patients with intestinal intestinal tissue in the amount of alpha-SMA,FAP,S100A6 and PDPN,SPARC is closely related to development of colorectal cancer.4、There are no significant differences between the two groups in terms of the expression of "the real" and "virtual" syndromes in the same group.