Effects Of MEBT/MEBO On The Expression Of PTEN/AKT/VEGF Signaling System In Chronic Wounds Of Rats

Objective: To further investigate the mechanism of MEBT/MEBO promoting the repair of chronic refractory wounds from the PTEN/AKT/VEGF signaling pathway.Methods: 90 adult SPF Wistar male rats,12 weeks old,weighing 220 g ~ 250 g,after 1 weeks of feeding,were randomly divided into blank group 18,control group(acute full-thickness group)18 rats,and chronic wounds in group 54.The rats in the blank group without any injury treatment;control group rats in the modeling of parts for full-thickness skin excision,but without the injection of hydrocortisone;After full-thickness skin excision in the molding parts,the rats in the chronic wounds group are injected the hydrocortisone to make chronic wounds models,then according to the treatment method were randomly divided into model group,MEBO group and b FGF group,18 rats in each group.Wounds in each group were treated immediately after making.On the third days,seventh day and fourteenth day after modeling,6 rats were killed in each group,take fresh wound tissue,immediately placed in liquid nitrogen tanks,and then quickly transferred to the-80 ultra low temperature freezer after the material is finished.After all specimens are collected,using WB and PCR method to detect the expression of PTEN?p-AKT?VEGFR2.Results:(1)Results of PCR:(1)The PTEN expression in granulation tissue of rats wound in MEBO group,b FGF group and control group changed with ime are showing a decreasing-increasing trend(P<0.05);Model group showed a decreasing trend(P<0.05);PTEN expression of three time points in MEBO group,b FGF group,control group and model group have significant differences(P<0.05);In the blank group,the rats tissue was normal skin,the expression of PTEN was not significantly changed due to the absence of injury treatment(P>0.05).(2)The VEGFR2 expression in granulation tissue of rats wound in MEBO group,b FGF group and control group changed with time are showing a increasing-decreasing trend(P<0.05);Model group showed a increasing trend(P<0.05);The VEGFR2 expression of three time points in MEBO group,b FGF group,control group and model group have significant differences(P<0.05);In the blank group,the rats tissue was normal skin,the expression of VEGFR2 was not significantly changed due to the absence of injury treatment(P>0.05).(3)The p-AKT expression in granulation tissue of rats wound in MEBO group,b FGF group and control group changed with time are showing a increasing-decreasing trend(P<0.05);Model group showed a increasing trend(P<0.05);The p-AKT expression of three time points in MEBO group,b FGF group and control group have significant differences(P<0.05);In the blank group,the rats tissue was normal skin,the expression of p-AKT was not significantly changed due to the absence of injury treatment(P>0.05).(2)Results of WB:(1)The PTEN expression in granulation tissue of rats wound in MEBO group,b FGF group and control group changed with time are showing a decreasing-increasing trend(P<0.05);Model group showed a decreasing trend(P<0.05);PTEN expression of three time points in MEBO group,b FGF group,control group and model group have significant differences(P<0.05);In the blank group,the rats tissue was normal skin,the expression of PTEN was not significantly changed due to the absence of injury treatment(P>0.05).(2)The VEGFR2 xpression in granulation tissue of rats wound in MEBO group,b FGF group and control group changed with time are showing a increasing-decreasing trend(P<0.05);Model group showed a increasing trend(P<0.05);The VEGFR2 expression of three time points in MEBO group,b FGF group,control group and model group have significant differences(P<0.05);In the blank group,the rats tissue was normal skin,the expression of VEGFR2 was not significantly changed due to the absence of injury treatment(P>0.05).(3)The p-AKT expression in granulation tissue of rats wound in MEBO group,b FGF group and control group changed with time are showing a increasing-decreasing trend(P<0.05);Model group showed a increasing trend(P<0.05);The p-AKT expression of three time points in MEBO group have significant differences(P<0.05);In the blank group,the rats tissue was normal skin,the expression of p-AKT was not significantly changed due to the absence of injury treatment(P>0.05).Conclusions:(1)MEBO can promote the healing of chronic wound in rats.(2)MEBO can reduce the expression of PTEN and PTEN m RNA in the granulation tissue of chronic wound.(3)MEBO can increase the expression levels of p-AKT,VEGFR2,p-AKT m RNA,VEGFR2 m RNA in granulation tissue of chronic wound.(4)MEBO may promote the wound angiogenesis by reducing PTEN,activating the AKT signaling pathway,and increasing the expression of VEGFR2 to promote the wound healing.