The Influence Of Medicinal Cake Partition Moxibustion On EMs Rats Model PTGS2 MRNA,KDRmRNA Expression And The Relevant Protein Content

Objective: This study proposed by comparing the efficacy of EMs model rats in Different groups and the influence on endometrial tissue PTGS2 mRNA,KDR mRNA and protein content.Delve into medicinal cake partition moxibustion medicine to the Da-zhui and Ming-men acupuncture point,the mechanism of action of EMs model rats treated.EMs for clinical treatment on the basis of the experimental study provides reliable and provide new ideas for the modern acupuncture clinical and new breakthrough.Methods: 50 healthy unfertilized female SD rats were randomly divided into blank control group,every medicine cake moxibustion group,gestrinone group,model group and control group,each group of 10.Observation group with every medicine cake moxibustion the Da-zhui and Ming-men acupuncture point;Drug group with gestrinone lavage;Model group and control group with normal saline lavage.Around 5 groups of rats before and after the treatment of ectopic endometrium growth situation,the endometrial tissue PTGS2 mRNA and KDR mRNA and its protein.VEGFA/KDR signaling pathway as the breakthrough point,in-depth study medicinal cake partition moxibustion treatment of endometriosis model rats and the effect mechanism.Results:?1?Compared with model group,gestrinone and everymedicine cake moxibustion groups of rats transplanted tissues can be observed narrowed,adhesion decreases,and with the surrounding tissue is relatively clear liquid in the vesicle,vesicle surface vessels to reduce?P < 0.05?;?2?The blank control group and control group of VEGFA,KDR,PGE₂ expression are low,this two groups of three indicators no significant difference?P > 0.05?.And the model group and treatment group two VEGFA,KDR,PGE₂ expression after building a significant rise.Found after treatment,compared with model group,every medicine cake moxibustion group rats VEGFA,KDR,PGE₂ expression appeared significantly reduced?P < 0.05??3?Building module rats PTGS2 mRNA and KDR mRNA expression is significantly higher than blank control group?P < 0.05?,the index control expression is no detail differences?P > 0.05?After treatment with separated medicine cake moxibustion,index expression of treatment group was obviously lower?P < 0.05?.Conclusions:?1?Interference VEGFA/KDR pathway or inhibit PTGS2 mRNA activity,can affect the development of endometriosis.?2?Medicinal cake partition moxibustion treatment of EMs effect may be mediated by VEGFA/KDR pathways.?3?The isolation effect the da-zhui and ming-men acupuncture point EMs curative effect is more significant,treatment green security,worthy of clinical popularization and application.