Treatment Of Dementia And Shichangpu From Sputum To Quasi - AD Rat Model The Mechanism And Mechanism Of The Study

ObjectiveTo investigate the pathogenesis and treatment of senile dementia (AD) from the perspective of phlegm, and to observe the effects of Acorus gramineus and its volatile oil on learning and memory, cholinergic system function, nerve growth factor and neuronal apoptosis in AD model rats And to explore the effect of Acorus gramineus on the AD model and the material basis for the prevention and treatment of AD for experimental basis.Method70 Wistar rats were randomly divided into 7 groups: normal group, sham operation group, model group, donepezil group(0.33mg kg-1 d-1), Acorus gramineus group (20g kg-1 d-1), Acorus gramineus decoction group (20g kg-1 d-1), ? -asarone group(25mg kg-1 d-1), 10 rats in each group. The hippocampus in rats were injected with A ? 1-42 to construct the AD rat model. Rats in each group were treated with 1ml/100g d for 28 days by means of intragastric administration. Morris water maze was used to observe the learning and memory ability of the experimental rats. The changes of Ach, AchE and ChAT in hippocampus were detected by radioimmunoassay. The NGF content in hippocampus was detected by ELISA and the apoptosis rate of neurons was detected by flow cytometry.Results1. In the 5-day navigation experiment, the escape latency of the rats in each group showed a decreasing trend, and there was no significant difference between the two groups before 2 days.On the third day, the escape latency of the model group was significantly longer than that of the normal group (P <0. 05).There was significant difference between the volatile oil group,the donepezil group and the 0 -asarone group and the model group (P<0.05). On the 4th day, the escape latency was significantly shorter than that of the model group (P <0.01);On the fifth day, the escape latency was significantly shorter than that of the model group (P <0. 01).2. The results of the 6th day exploration experiment showed that the percentage of swimming time in the model group was significantly shorter than that in the normal group, and the spatial search ability decreased significantly (P<0.01). The ratio of quadruple decoction group, Acorus gramineus decoction group, Acorus gramineus volatile oil group and ?-asarone group was significantly higher than that of model group (P>0. 05). The number of rats in each group after crossing the original platform showed that the number of rats in the model group decreased significantly compared with the other groups (P<0.01) ). The number of times of donepezil, Acorus gramineus decoction group, Acorus gramineus volatile oil group, ?-asarone group and model group was significantly higher than that of the original platform (P <0. 01).3. Compared with normal group, Ach and ChAT were significantly decreased in model group (P <0.01), while AchE activity was significantly increased (P <0.01). Compared with the donepezil group, the Ach and ChAT contents of Acorus gramineus decoction group were significantly different (P<0. 01),and there was significant difference between the two groups(P<0. 05) . Compared with the donepezil group, the AchE activity of Acorus gramineus decoction group and ?-asarone group was significantly different (P<0.01), but there was significant difference between the two groups (P>0. 05, P <0.01).4. The content of NGF in hippocampus showed that the content of NGF in model group was significantly decreased (P<0. 01). The results of NGF in the treatment group were significantly higher than those in the control group (P<0.01). Compared with the donepezil group, the contents of NGF in the Acorus gramineus decoction group, the volatile oil group and the ?-asarone group were significantly different (P<0.05, P <0. 01).5. Flow cytometry was used to detect the apoptosis of hippocampal neurons in rats. It was found that the peak type of hypodiploid cell population appeared on the left side of G1 peak in neurons of model group. The diploidy of the treatment group appeared to some extent. The apoptotic rate of hippocampal neurons in the model group was significantly higher than that in the normal group (P<0.01). Compared with the model group,the apoptotic rate of the neurons in the treatment group was significantly lower than that in the control group (P<0.01).Compared with the donepezil group, the rats were treated with Acorus gramineus decoction group, Acorus gramineus volatile oil group and ?-asarone group The apoptotic rate of nerve cells was significantly different (P <0.05, P <0.01).Conclusion1. Phlegm obstruction and blind the brain are important factors for dementia,phlegm resuscitation and awakening is an important rule of dementia treatment.2. The treatment group can effectively improve the learning ability of the experimental rats in terms of the navigation test that reflects the ability of spatial learning and memory acquisition, or from the spatial search experiment which reflects the information storage ability. Among them, the volatile oil group was the best, followed by the order of donepezil group, ?-asarone group and Acorus gramineus decoction group.3. The damage of Acorus gramineus and extract on the central cholinergic has a repair effect, so as to achieve the purpose of improving learning and memory, which Acorus gramineus volatile oil group the best results.4. Acorus gramineus has the effect of inhibiting the apoptosis of brain neurons and increasing the content of endogenous NGF, and the two are closely related. Suggesting that Acorus gramineus can increase the content of endogenous NGF, thereby inhibiting the occurrence of apoptosis, and promote the amount of cholinergic fibers in the denervation region, which contributes to the rapid recovery of neuronal injury. Its specific mechanism of action to be further explored.