| ACOrus tatarinowii schott. as a broadly used herb in clndcs,is widely aPPlied to the ePilePSy, PhlegIn -induced synCope,unconsciousness caused by febrile diseases, amnesia, aPhemiacaused by wind strOke, tinnitUS and senile forentia, and it isone of the few good herbs for ccrebral diseases.This stody mainlyaims at exPoring the fUnction of Acorus tatarinowii schOtt tO thecental neUIa syStCm, its atrial base fOr the boon and theftmchonal mechasm. Its puopse is tO Provide scientific baSisfOr fwther developing Acorus tabonowii schOtt.Objective1. TO explore the Inaterial base of Acorus tatarinowh schOtt tbritS thechon tO the central neural system and Pthearily ideIItifyits effodive PartS. by deriving different ghs from AcorusWnowii schOtt and making synthetc analysis with thediffercnt perts of the intemal and extemal ingrwhents of thebomal's brain tissue.2. To Observe the effeCt of the diffetal op of AcorusHnowii schOtt on the mouse's convulsing latCnt period, itsconvulsing frequency, itS onset frequency of cOnvulsion. deathincNon and its mortality with a view tO exPloring tbePharInacological M of the hation of Acorus tatarinowiischOtt to central nend Sydem(CNS)3. To Observe the effect of the differen Parts of AcorusWnowii schOtt on the beion of convulsion of themouse and convulsion occurrenee nd so as tO furthur confirmthe funCtion of Atorus tatarinowii schOtt to CNS byexperimenting the effect of Acorus tatarinwii schOtt on themouse of the convulsion caused by sodium glUtamate.4. TO observe the effbet of the different Parts of AcorusWnowii schott on aznpltale and frequellCy of5eleCtrOCnCePbalOgraIn of the rat in order tO Prove thc direCfunction of Acorus tatarinowii schOtt tO CNS byexperimenting its effect on electrOecePhaogram.5. To exPlore the hat1on pointS of the differen pe Of Acoruswtamli SCho tO CNS, by testing edces Of SOD .LPO, NO GABA.Gu. AsP, Gy. all and Ta4 etc.Mathodsl ExtraCing the volatile oil and the decoction of the de-fatting ofAcorus tatarinowii schOtt respehvely by distilling method andcircumnuencc method, and ndng analysis with andmensUfating the ingredcnts of the differen pe of Acorustatarinowii inside and outside thc mouse's brain by GCAlSmethod.2.Observing the drug effeCt of differmt PartS of Acorustatarinowii thOugh makjng an convulsing mouse's model byinjecting strychnin solulion into the mouse's bomen cavityThe sollltion is used tO excite the spinal cord.3.Further obsehang the drug effect of the different pe ofAcorus tatarinowii schOtt thrOugh Inaha an cOnvulsingmouse's mOdel by injeCtin sodium glUtamate solution into themouse's IatCral ventricle. The SOation is used tO excitC thebrain.4.Looking into the effect of the different pes of ACorustatarinowii schott on elcctrbenCePhalogram and cording therat's eleCtrOe~opo using bioinstrUmenotion by directasuMtUre electrode and whPOlar lead method.5. Exploring the mechanism of the funcion of the different pesof Acorus tatarinowii schott tO CNS thrOUgh testing LOP,SOD, NO and excitatory atmitter of amino acidsresPeCtively by TAB method, borhol aotic okidationmethed and nitric aGd reducthe method, and mcnsedngamino acids neurotransmiher such asGABA, Gl u AsP Gly Ala,Tau by HPLC method.6Resultsl. The analysis of cheIniGal ingredients of differen Parts ofAcorus tatarinowii schott and of the ingredents which haveenter the animai's brain tissue.l.l The analysis of chemical ingrdientS of different Prt ofAcorus tatarinowii schOttGC-MS analysis shOws tha the han ingredients of thevolatile oil of Acorus tatarinowii schOtt are cis-isoeugenol methylether,hanS-i socugen ol -methyl ether,acoradiene, e lemi ci n, P--asarone, Q --asarone The main ingrediellts of decOCtion arecis-isoeugenol methyl, ether, tranS-isoeugeno l-methyl other, D--asarone, and the main ingrdient of the deodion of thede-fatting of AcorUS tatarinowii schott is D --... |
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