The Research On The Regeneration Of Rabbit Skull Defect Of Pangasisus Haniltoa Skin Collagen Scaffold

Objective To screen out the best properties of Pangasisus haniltoa skin from several common skin skins as a raw material source for the material.A membrane-like material with the main component of collagen was prepared by freeze-dried Pangasisus haniltoa skin.The effect of the membrane on the bone marrow regeneration was analyzed,and the effect of the membrane on the bone regeneration was analyzed.The effect of the membrane on the regeneration of the barrier membrane was analyzed.The collagen membrane was used as the bone membrane applied to the clinical basis and the possibility to explore whether the Pangasisus haniltoa skin can become a new source of collagen scaffold material.Method In this paper,the constituents of collagen extracted from Pangasisus haniltoa of skin were analyzed.Pangasisus haniltoa skin after repeated degreasing decolorization and other treatment,and finally freeze-dried film-like.The physical and chemical properties of the membrane were studied.The morphology of the membrane was observed by scanning electron microscopy,the structure of the material and the size and distribution of the pores.The porosity of the collagen film was determined by liquid displacement method.Determine the mechanical strength of the material.The in vitro degradation rate of the membrane was determined according to the weight loss rate after 6 hours of soaking in phosphate buffer.The water absorption of the film was measured and the swelling of the film in a wet state was observed.The denaturation temperature of the material is determined by the change in viscosity due to temperature changes.CCK-8 cytotoxicity test to determine whether the material is toxic to the organism.The effect and potential of the membrane as guide bone regeneration membrane were preliminarily determined by the bone regeneration experiment of rabbit skull defect.Results The content of crude protein was 95.20%,and the collagen type was mainly type I collagen.Compared with other fish,Pr O and Hyp contained in the collagen of Pangasisus haniltoa skin were the highest in all amino acids.Thickness uniform.Scanning electron microscopy shows that the material material is a two-layer structure,one side is loose and porous,the pore size is different,the distribution is uniform;the other side is smooth and the pores are dense.Porosity(55.50 ± 1.94)%,thickness(0.66 ± 0.10)mm,tensile strength(18.82 ± 0.94)MPa.The degradation rate of the material was(80.22% ± 2.49)for 28 days,and the water absorption at 48 h was(379.77% ± 77.81).The denaturation temperature of collagen film is about 34 ?,and the denaturation temperature of Pangasisus haniltoa skin after DHT can be increased to about 35 ?.In the rabbit skull defect regeneration experiment,Pangasisus haniltoa skin collagen membrane can play a role in guiding bone regeneration in the defect area and skin and mucous membranes and other soft tissue barrier,effectively protect the defect area,blocking the fibrous cell growth,The bone process provides a stent that promotes the vascularization within the regenerated tissue.The results of the final analysis can be found in Pangasisus haniltoa fish skin collagen film osteogenesis effect better than the blank control group,and Bio-Gide membrane group almost no difference.Conclusion Pangasisus haniltoa skin collagen film physical and chemical properties of stable,the surface has a certain porosity,and for the double structure,one side rough,with more pores,smooth and dense side.Water absorption is moderate,no significant deformation and dissolution after water absorption.Stability is good,the degradation rate is higher than that of Bio-Gide collagen membrane.No cytotoxicity,with good biocompatibility.In the rabbit skull defect surgery can play a role in guiding bone regeneration.This material has the potential for further research and development,and has the possibility of becoming a new type of clinical medical membrane material.