Pharmaceutical Study Of Feishu Oral Liquid

Feishu oral liquid is empirical formula of Yan Xiaocheng director of China academy of traditional Chinese medicine, which is a compound preparation of traditional Chinese medicine consisting by lonicerae flos?portulacae herba?rhei radix et rhizoma and glycyrrhizae radix et rhizoma. It has the efficacy of clearing heat and purging fu organs, cough expectorant. For evil heat invading the lung caused by lung heat cough, symptoms such as coughing, have fever, sputum yellow sticky, dry mouth, sore throat, headache, dry stools and so on, that is acute upper respiratory tract infection, influenza, acute chronic bronchitis, pneumonia and other respiratory diseases and acute pharyngitis, acute tonsillitis and pharyngeal diseases disease. Under the guidance of traditional Chinese medicine theories and with the help of the modern pharmaceutical technology, this study is on its preparation process?quality standards and standard fingerprint.According to the function of the prescription and the nature of drug ingredients, by using overall desirability as screening index, the best extraction process and condition were screened with orthogonal test method:For the first time, with 12 times water, add 2.6 times water after soaking 2h, extracting 30min. The second and the third with 12 times water, extracted 30min each time. In clarifying the purification process, with comparison of methods of centrifugal, ethanol precipitation, chitosan flocculation, gelatin flocculation, etc, the best clarification and purification process were screened with central composite design-response surface Methodology:Liquor is decompressed and concentrated (temperature:80?, the vacuum degree:-0.04? 0.06MPa) to a crude drug: liquid (1:3), adjusted to pH 4.7, adding 1% chitosan containing chitosan amount to 0.1%, while rapid mixing, cold storage standing 24 hours. By study of concentrating density, pH, preservative, it is determined purification solution should be concentrated to 1.15?1.20(40?) and pH range of products should be from 4.0 to 5.5, and add 0.1% potassium sorbate as a preservative. Thermodynamically stable system study proves that the oral system is stable.Establish a quality standard of Feishu oral liquid. the products characteristics, examination were in line with regulations. Set up the feasible TLC methods for the identification of lonicerae flos?portulacae herba-. rhei radix et rhizoma and glycyrrhizae radix et rhizoma. establish an HPLC method for the chlorogenic acid determination, stipulate products no less than 55.75mg per 10mL.Qhenomenex Prodigy ODS3(250x4.60mm,5?m) chromatographic column, with 0.4% CH3COOH-acetonitrile as the mobile phase for gradient elution. The detection wavelength was 254 nm, and the flow rate was 1.0mL-min-1. The column temperature was 30?. Initially established Feishu oral liquid fingerprints, and find lonicerae flos?portulacae herba?rhei radix et rhizoma and glycyrrhizae radix et rhizoma correlation with peaks in fingerprints.Studies have shown that the preparation process is reasonable and feasible, quality standards is controllable, fingerprints have special properties that can be used for quality control in the product.