| The increasing seriousness of land desertification, make the desert control is imminent, and research on how the sand is also more and more get the attention of people, and psammophyte occupies very important position in the governance of desertification. If can effectively develop psammophyte economic value, can drive the enthusiasm of people grow, so as to improve the efficiency of the desert governance, to achieve the sustainable development of desert prevention and cure. Pugionium as a kind of medicine and food with psammophyte, belong to the dominant species in the desert vegetation, and can be very good to adapt to the dry desert environment, occupies an important position in the desert governance. Though, a lot of research on sand mustard, but mainly concentrated in the botany characteristics and apparent research, and less research about its function. This paper for the first time at the cellular level and organizational level by in vitro experiments on sand mustard extract to adjust the action of gastrointestinal dynamics is studied, the preliminary explore its possible mechanism of action, and for the first time, the active compounds were isolated. The Pugionium theoretical basis is provided for the development of the medicinal value, it is of great significance.The main research results of this paper is as follows:1. By in vitro experiment, adopting the gastric smooth muscle cells and colon smooth muscle contraction to detect sand mustard extract on gastrointestinal dynamic adjustment.The experimental results show that the EEP can relax the stomach smooth muscle cells, when the concentration of EEP was 200mg/ml, the diastolic rate was 23.51%, and have a does-response relationship in the context of 40mg/ml-200mg/ml. EEP can inhibit the natural contractions of rat colonic smooth muscle, and have a does-response relationship between 1 mg/ml-200mg/l,the relative IC50 was 99.42+ 0.16mg/ml; After extracting the EEP found only the EAEP has the SMC diastolic function, the relaxation rate is 16.64%. The EAEP inhibit colonic smooth muscle contraction and have dose-effect relationships within the 10mg/ml-100mg/ml, the IC50 value is (46.91±0.27) mg/ml, relative IC50 decreased 52.8% than that of EEP.2. We studied the effect of EAEP on concentration of calcium ion in SMC, HEK293 and HEK293/MLNR found that the EAEP can increased concentration of calacium ions in the SMC, HEK293 and HEK293/MLNR, but the irritation of HEK293/MLNR was significantly lower than that of HEK293. EAEP can effectively improve the calcium ion concentration of the gastric smooth muscle cells, have a does-response relationship in the context of 20mg/mL-100mg/mL.In the experiment of effect of EAEP on colon smooth muscle contractile, we found that EAEP could inhibit acetylcholine-contraction of colonic smooth muscle strips, and the inhibition was 35.57%±3.3%. After of pretreatment with indomethacin and methlylene blue, the inhibition rate of EAEP on smooth muscle was 11.24%±8.2% and 33.64%± 5.5%, it was significantly reduced compared with the control group(61.17%±3.3%). It is therrfore suggested that the relaxing effect of pugionium cornutum attributes to the interaction of GC and COX stimulation, as well as the antagonism effect on acetylchohne, and regulation of pugionium cornutum on gastrointestinal motility related to intracellular calcium ion concentration.3. Separation by silica gel column chromatography and HPLC method, combined with the smooth muscle contraction, the compound P-2-1 was isolated. It was identified as quercetin by mass spectrometry, nuclear magnetic, ultraviolet and infrared spectrum testing. |
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