| Two Macrocephala Flavored Powder is the preliminary experiment according to weaning piglets in veterinary population causes dialectical and self in Chinese medicine powder. In this study, based on the test, the Two Macrocephala Flavored Powder was made into granules, and its pharmacodynamic study. The specific test methods and results are as follows:1.Two Macrocephala Flavored Granule Screening of Two Macrocephala Flavored Granule preparation process:Single factor investigation of Two Macrocephala Flavored Granule extraction, according to the requirements of the veterinary drug standard of the Peoplr's Republic of China (second) detection of the content of each ingredient with HLPC. To detect the content of each ingredient in each method with Orthogonal experimental model, and determine the best extraction process. The last, according to the HPLC method for the determination of the content of the granules made of each kind of auxiliary materials, the auxiliary materials were determined.2. The establishment of diarrhea model and the effect of theTwo Macrocephala Flavored Granule treatment:According to reference and two Macrocephala Flavored Powder's medication principle "inhibiting wood and strengthening earth, promoting qi activating blood ",choose the Folium Senna to establishment of "liver stagnation and spleen deficiency" diarrhea model. The mice treated with Senna Decoction, once a day,5 days in a row. from the second day began to kill the mice, to observe the shape of intestinal contents in mice. According to the mouse diarrhea score table to score the degree of diarrhea, determines whether the model is successfully. Based on the successful establishment of diarrhea model. Using two methods of treatment and prevention to observe the effect of Two Macrocephala Flavored Granule treatment on drug action. The treatment group first treated with Senna decoction for two days, and then treated with Two Macrocephala Flavored Granule, in 2H,4h,6h,8h four time point to kill the mice; The prevention group was first filled with Two Macrocephala Flavored Granule for three days, and the treated with Senna decotion, in 2H,4h,6h,8h four time point to kill the mice. Observe the intestinal contents of mice were killed and detect the content of mucosal lactase, to determine the effect of the medicine on the medicine of Two Macrocephala Flavored Granule.3. Study on pharmacodynamics of Two Macrocephala Flavored Granule:To determine the effect of Two Macrocephala Flavored Granule drug action, to study the pharmacodynamics. Setting three dose groups, High, medium and low group. To the mice with the corresponding dose of Two Macrocephala Flavored Granule three days, then treated with Senna decoction, in 2H,6h,8h four time point to kill the mice. According to observe the intestinal contents of mice, to detect the content of mucosal lactase, amylase and trypsin,and observation of intestinal tissue sections to To determine the degree of diarrhea in mice. Determination of IL-1? and TNF-ain serum of mice, NHEs mRNA expression to observe Two Macrocephala Flavored Granule treatment mechanism.The result:The Two Macrocephala Flavored Granule's optimum extraction process is extraction time 1H, extraction times of 2 times,15 times the amount of alcohol, alcohol concentration 50%, accessories for the screening of lactose. After the preparation of the Two Macrocephala Flavored Granule, the transfer rate of the four traditional Chinese medicine can reach more than 80%. The diarrhea model was established on the second day of the mice and the diarrhea rate reached 100%. Fourth days to start the phenomenon of self healing, the diarrhea model established successfully and in second to third days of diarrhea was the most serious. The curative effect of prevention group was better than treatment group (P<0.05) with the degree of diarrhea and mucosal lactase content. Therefore, the use of preventive methods to the Two Macrocephala Flavored Granule for pharmacodynamic evaluation study. In pharmacodynamic evaluation test. The content of mucosal lactase in mice in drug group were higher than that of negative control group (P<0.05). The amylase content in the drug group was lower than that of the negative control group (P< 0.05). with the change of time, there was no difference between the high dose group and the positive control group. The trypsin content in the drug group was lower than that of the negative control group (P< 0.05). The traditional Chinese medicine group IL-1? is lower than the negative control group (P<0.01). the traditional Chinese medicine group TNF-a is lower than the negative control group(P< 0.05).The traditional Chinese medicine group was higher than that of the negative control group in the 3 time period(P<0.05). The crypt depth of traditional Chinese medicine group was lower than that of the negative control group (P<0.05). The traditional Chinese medicine group V/C was higher than the negative control group (P<0.05). The expression of NHE2, the Chinese medicine group were higher than the negative control group and the positive control group (P< 0.05). The expression of NHE3 in traditional Chinese medicine group was higher than that in negative control group and positive control group (P< 0.05). The expression of NHE8 in high dose group was higher than that of negative control group and positive control group (P< 0.05). The middle dose group and low dose group were higher than the negative control group (P <0.05), and the positive control group was not significantly different.The conclusion:Two Macrocephala Flavored Granule has obvious therapeutic effect on diarrhea in mice. Two Macrocephala Flavored Granule to reduce the content of proinflammatory factors. The expression of NHE2 in the traditional Chinese medicine group was higher than the negative control group (P<0.05). The expression of NHE3 in traditional Chinese medicine group and negative control group were not significant. The expression of NHE8 in high dose group of traditional Chinese medicine group was higher than that in negative control group (P<0.05)? It is possible to treat the diarrhea of mice by regulating the expression of inflammatory factors and mRNA NHE. |
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