The Effect Of Imatinib Mesylate On Complicance Of Neurogenic Bladder Caused By Spinal Cord Injury On Rats

Objective:The aim of this experiment is to establish a stable rat suprasacral spinal transection model of neurogenic bladder. We observed the urodynamic parameters include bladder capacity,detrusor leak point pressure and bladder compliance, measuring the bladder weight change, and we also observed the changing of urodynamic parameters after intravenous injection administration of Imatinib mesylate after 12 weeks of spinal cord transection. We want to investigate the changing of bladder compliance and pathophysiology after injury. We look forward to discovering the involving mechanism of ICC-like cells in the pathogenesis of neurogenic bladder, and providing some experimental basis for further study, especially the pathogenesis of low compliance bladder caused by spinal cord injury. We also want to provide a theoretical basis for non-surgical treatment of neurogenic bladder.Methods:A total of 30 female Wistar rats were randomly divided into two groups: Group A was sham group of 12-weeks,had 8 rats. Group Bwas suprasacral spinal cord transaction group of 12-weeks,had 22 rats. Spinal cord transectionat T10 level was built in group B, whilegroup A underwent sham operaion. The main steps are as follows: the rat was weighed and fixed on board after anesthetized, and then navigated T10 spinal segments, spinal cord was exposed andtransectedby ophthalmic scalpel.Rat into sham groupswas only exposed T10 spinal cord, and then sutured incision. Urodynamic were detected in 12 weeks after surgery. Group A and groupB urodynamic detected again after intravenous injectionadministration of Imatinib mesylate. Bladders of all groups were weighed after cystometry.Results:1. We successfully established a stable rat suprasacral spinal transection model at T10 level.2. After 12 weeks of surgery, there were 15 rats survived in the group B.All ratssurvived in group A. The urodynamic results showed thatthe bladder compliance ofgroup B was significantly lower than group A(P<0.05).3. The bladder weight increased(P <0.01); the bladder / weight ratio of the experimental groups were higher than the control group(P <0.01).4. Imatinib mesylate(5 umol) improved bladder compliance group B(P <0.001) compared to that of pre-administration. Imatinib mesylate(20 umol) improved bladder compliance group B(P <0.001) compared to that of pre-administrationand Imatinib mesylate(5 umol) be administrated. There was no significant change to the bladder compliance of group A when the Imatinib mesylate(5 umol) be administrated. Imatinib mesylate(20 umol) improved bladder compliance of group A(P <0.001) compared to the controlConclusions:1 We reported that the bladder compliance of the T10 spinal cord injury rats increased at 12 weeks.2 The urodynamic performance of the neurogenic bladder caused by spinal cord injury in ratsshowed that,in the late stage, the bladder maximum capacity higher than in the control group, and the detrusor leak point pressure significantly increased but the bladder compliancereduced.3 Imatinib mesylate improved bladder capacity, compliance, and reduced the detrusor leak point pressure. The effect of Imatinib mesylate provides a theoretical basis for non-surgical treatment of improve the neurogenic bladder compliance.