Study On The Expression Of Adiponectin, VEGF, MMP-9 And Their Correlations In The Breast Cancer

Objective:This study aimed to test the expression of adiponectin, VEGF and MMP-9 in breast cancer and their relationship with clinical pathological characteristics. Methods:1. 40 cases of paraffin-embedded breast cancer tissues treated in Tianjin Medical University Cancer Institute and Hospital from 2013 December to 2014 August, and 40 cases of normal adjacent tissues were collected. The expression of adiponectin, VEGF and MMP-9 were detected by immunohistochemistry. The distribution of adiponectin, VEGF and MMP-9 were investigated, and the correlation between above 3 makers and clinical characteristics was evaluated.2. MDA-MB-231 cells were cultured with varid concentration of adiponectin. The expression of VEGF and MMP-9 was detected by Western blotting. We evaluate the influence of adiponectin on the expression of VEGF and MMP-9 in vitro. Results:1. The expression of adiponectin in breast cancer tissues and normal breast tissues were 57.5% and 92.5% respectively. we found the expression of Adiponectin was lower in breast cancer, and the difference had statistical significance(P =0.001). Lower expression of adiponcetin correlated with the larger tumor volume, more advanced histological grade, displayed negative correlation with the metastatic axillary lymph node. However, there are no significant correlations among adiponectin, age, length of the course of the disease, clinical stage, and the expression of ER, PR and C-erb B-2, and so on(P > 0.05).2. The expression of VEGF in breast cancer tissues and normal breast tissues were 52.5% and 15% respectively. we found the expression of VEGF was higher in breast cancer, and the difference had statistical significance(P=0.001). Lower of expression of adiponcetin correlated with the more advanced histological grade, displayed negative correlation with the metastatic axillary lymph node. However, there are no significant correlations among adiponectin, age, length of the course of the disease, tumor volume,clinical stage, and the expression of ER,PR and C-erb B-2, and so on(P > 0.05).3. The expression of MMP-9 in breast cancer tissues and normal breast tissues were 55% and 10% respectively. The expression of MMP-9 was higher in breast cance, and the difference had statistical significance(P < 0.05). Lower of expression of adiponcetin correlated with the more advanced histological grade, displayed negative correlation with the metastatic axillary lymph node. However, there are no significant correlations among adiponectin, age, length of the course of the disease, tumor volume,clinical stage, and the expression of ER,PR and C-erb B-2, and so on(P > 0.05).4. In breast cancer, the level of diponectin was negatively correlated with the expression of VEGF and MMP-9.5. In MDA-MB-231 cells, along with increase of the concentration of adiponectin increased, the expression of VEGF and MMP-9 decreased, which implied that adiponectin might inhibite the expression of VEGF and MMP-9. Conclusion:1. Compared with the normal breast tissues, the expression of Adiponectin was lower in breast cancer tissues. The expression of Adiponectin was negatively associated with tumor size, histological grade and axillary lymph node metastasis.2. Compared with the normal breast tissues, the expression of VEGF was higher in breast cancer tissues. The expression of VEGF was positively associated with histological grade and axillary lymph node metastasis.3. Compared with the normal breast tissues, the expression of MMP-9 was higher in breast cancer tissues. The expression of MMP-9 was positively associated with histological grade and axillary lymph node metastasis.4. Expression of adiponectin, VEGF and MMP-9 in breast cancer tissue was negatively correlated.5.Adiponectin might inhibite the expression of VEGF and MMP-9.