Research On Analysis, Extraction And Purification Of Medicinal Active Ingredients In Flos Lonicerae

Flos Lonicerae is a traditional Chinese medicine, belonging to caprifoliaceae plants. It has the functions of clearing away heat and toxin, resisting disease, strengthening the immune function, central excitation, reducing blood fat, resisting endotoxin and so on, and It contains amino acids, nucleotides, organic acids, iridoid compounds, chemical constituents of flavonoids and alcohols etc. In this paper, the medicinal activity —— chlorogenic acid and flavonoids in Flos Lonicerae were taken as the research object, for analysis, extraction and purification study.Various kinds of existing analysis methods were analyzed. The results showed that the Correction direct method which was established in this paper could be used for the simultaneous analysis of chlorogenic acid and flavonoids in Flos Lonicerae conveniently and directly.The extraction, reflux and ultrasonic extraction method were compared and analyzed, the results show that the room temperature extraction method can effectively extract chlorogenic acid and flavonoids, without damaging the structure. Using single variable method to optimize the extraction process of extraction of active ingredients from Flos Lonicerae, take 70% ethanol solution extraction, solid-liquid ratio of 1:12, extraction time of 1.5h for the best extraction conditions, the extraction rate of chlorogenic acid was 5.5%.Using macroporous resin and HSCCC to separate and extract the active constituents from Flos Lonicerae. The volume of 4 ethyl acetate was selected for the separation of chlorogenic acid and flavonoids. Using D101 macroporous resin, with a rate of 2ml/min sampling and elution: optimum conditions in the purification of chlorogenic acid in Flos Lonicerae of adsorption is the static adsorption time 2h, sample pH is 3, 70% ethanol as eluent. New phase separation process was then used for further purification, n-hexane as phase separation agent, dosage for ethyl acetate extraction agent of 0.4 times the ethyl acetate solution as extractant; optimal conditions of purification of D101 macroporous resin on the adsorption of total flavonoids in Flos Lonicerae is static adsorption time 2h, eluent pH 2, 50% ethanol solution as the eluant and the eluate was recrystallized. Through this process, the purity of chlorogenic acid was 72.8%, while the purity of the total flavonoids was 51.5%. Selection of ethyl acetate: ethanol: water(3:1:4, V/V/V) as the solvent system with HSCCC, the detection wavelength was 254 nm. chlorogenic acid and flavonoids were isolated from the ethyl acetate layer, and the purity of chlorogenic acid was 89.12%, purity for the total flavonoids was 75.48%.