Study On Octenidine Dihydrochloride Loading Titanium And Its Antibacterial Property

Titanium and its alloys were widely used as implants in orthopedic, dental and plastic surgery fields, mainly owing to their good biocompatibility and mechanical properties. Bacterial infection is one of the most severe postoperative complications leading to implantation failure. The early stage period(4- 6 h) was confirmed to be the “decisive period” for long-term bacteria-related infection. Octenidine dihydrochloride(OCT), as a new antibacterial agent, possesses numerous advantages, such as good tissue compatibility, broad antibacterial spectrum, fast action, long-term function and satisfactory stability etc. This study aims at endowing Ti-based materials antibacterial property by loading antibacterial agent of OCT via electrostatic adsorption, which maintaining the cytocompatibility of titanium-based materials and simultaneously inhibiting bacteria adhesion. Firstly, titanium substrates were pre-treated with 5 % H2O2 for 24 h. And then the treated Ti substrates were immersed into OCT solutions with different concentrations(1 ?g/mL, 5 ?g/mL or 10 ?g/mL) at room temperature for 24 h, so as to optimize the loading process of antibacterial agent. The study performed the drug release experiments in PBS at 37°C. The results confirmed that an average release about 62% of OCT from three titanium substrates at the early stage of 6 h, which is essentially important for bacterial inhibition in potential application.The in vitro cytocompatibility tests including CCK-8, ALP and mineralization verified that 5 ?g/mL OCT loading Ti-based substrates(about 1 ?g/disc) caused no adverse effects on the proliferation and differentiation of osteoblasts after culture for 4, 7 and/or 14 days. Meanwhile, scanning electron microscopy observation confirmed that the titanium substrates had strong early antibacterial capacity against both S. aureus(gram-positive) and E. coli(gram-negative) at the “decisive period” of 6 h. Overall, this study provides a promising approach for the fabrication of functional titanium implants, which could inhibit the early bacterial adhesion and maintain the biological functions of osteoblasts.