Study On Preparation Of Nano-mesh On Titanium Surface And Its Biological Evaluation

Titanium(Ti) and its alloys were widely used in clinical orthopedic and dental fields due to their excellent mechanical properties and bio-compatibility. However, Ti implants are usually failed in clinical because Ti has poorly osseointegration and is easily to be infected by bacterials. Moreover, as an implant material, the micro-structure on implant surface has high surface roughness and can increase the area with the surrounding tissues, which can improve the bio-compatibility. So, surface functionalization of an implant is one promising alternative for improving osseointegration and/or reducing bacterial infection.In this study, nano gel mesh structures, was constructed on Ti surface by spin-coating and alkali-heat treatment. Surface topography has been identified as a crucial property that affects osseointegration, thus the method of topographical modification was frequently adopted in Ti implant research. Zinc is one of the necessary trace elements in human body. Zinc ions at appropriate dose have positive impact on cells growth. Firstly, through a series of conditions optimization, we constructed nano gel mesh structures on Ti surface by spin-coating and alkali-heat treatment. Then, these structures was characterized by SEM?XRD?XPS and ICP. Secondly, we demonstrated the promotion of osteoblasts differentiation via cells experiments. After incubating for 4 and 7 days, osteoblasts on Ti-Zn0.3 displayed significantly higher cell viability and ALP activity(p<0.01) than those on pure Ti. The morphology of osteoblasts were characterized by CLSM, and the images showed that Ti-Zn0.3 were more beneficial for osteoblasts to adhere and spread. After 7 and 14 days, osteoblasts on Ti-Zn0.3 displayed significantly higher mineralization ability(p<0.01) than those on pure Ti substrate. Our study demonstrated the zinc-incorporated coating has a good biocompatibility in vitro. It indicated that we prepared structure on titanium surface has good clinical application prospects. Finally, we choose two kinds of common bacterials, Escherichia coli and Staphylococcus aureus for antibacterial assay. The results indicated that zinc-incorporated structures substrates could effectively inhibit the growth of bacterials.