Association Of Polymorphisms In The MIF Gene (173G/C) And IL-17 Gene (rs2275913,rs763780) With HBV Infection In Qingdao Han Population

Objective:To investigate whether there is an association of polymorphisms in the MIF gene(173G/C) and IL-17 gene(rs2275913, rs763780) with susceptibility to HBV infection in a Chinese Han population of Qingdao.Methods:(1) HBV infection cases and controls matched for age from Chinese Han population of Qingdao were recruited in this study. Genotyping of MIF-173G/C polymorphism was performed by allele-specific polymerase chain reaction. Genotyping of IL-17 A rs2275913 and IL-17 A rs763780 polymorphism was performed by a taqman-based real-time PCR. The frequencies of the alleles and genotypes in patients and controls were compared by the ?2 test.Results :(1) MIF gene-173 G/C polymorphism analysis results: The frequencies of CC,GC, GG genotype in HBV infection patients were 4.7%, 31.2% and 64.1%, respectively, while in the controls thosewere 2.9%, 28.0% and 69.1%. There was no statistical difference between MIF-173G/C genotype frequencies of HBV infection groups and controls. The allele frequencies of C and G in HBV infection groups were 20.3%and 79.7%, while in the controls those were 16.9%and 83.1%, and significant differences between two groups were found(P=0.032, OR= 0.799, 95%Cl=0.651-0.981). Among them, genotype CC, GC, GG frequencies were 5.5%, 31.7% and 62.8% in male HBV infection groups, while those were 3.4%, 28.9% and 67.7% in control groups. The allele frequencies of C and G in male HBV infection groups were 21.4%and 78.6%, while those were 17.8%and 82.2% in the controls groups. There was no significant difference in terms of the frequencies of alleles and genotype between the two groups of male. The frequencies of CC, GC, GG genotypes were 3.3%, 30.2% and 66.5%in female HBV infection patients, while those were 2.2%, 26.3% and 2.2%in the controls. The allele frequencies of C and G in female HBV infection groups were 18.4% and 81.6% respectively, while in the controls those were 15.4% and 84.6%. There was no significant difference in terms of the frequencies of alleles and genotype between the two groups of female.(2) Analysis results of the genotypes of IL-17 A rs2275913: The frequencies of AA,AG, GG genotypes were 19.3%, 30.6%and 50.1%, in HBV infection patients, while those were 22.4%, 44.0%and 33.6%in the controls. There was significant statistical difference in IL-17 A rs2275913 genotype frequencies between patients and controls(x2=36.89, P<0.001). The allele frequencies of A and G in HBV infection groups were 34.6%and 65.4%, while in the controls those were 44.4%and 55.6%, and significant differences between them were found(P<0.001, OR=0.645, 95%Cl=0.540-0.770).(3) analysis results of the genotypes of IL-17 F rs763780: The frequencies of CC,CT, TT genotypes in HBV infection patients were 0.7%, 10.2%and 89.1%, respectively, while those were 1.2%, 19.3%and 79.5%, in the controls. There was significant statistical difference in IL-17 F rs763780 genotype frequencies between patients and controls(x2=19.80, P<0.001). The allele frequencies of A and G in HBV infection groups were 5.8%and 94.2%, while in the controls those were 10.9%and 89.1%, and significant differences between them were found(P<0.001, OR=0.507, 95%Cl=0.371-0.692).Conclusion: These results indicate that MIF-173 G/C, IL-17 A rs2275913 and IL-17 F rs763780 are associated with HBV infection of han population in Qingdao area, the MIF-173 C allele, GG genotype and G allele of rs2275913, TT genotype and T allele of rs763780 might increase the risk of HBV infection. All of these results were first discovered.