| Objective To evaluate the protective effect of recombinant human erythropoietin on intestinal tissue of sepsis rats preparated by cecal ligation and puncture and its possible mechanism.Methods 36 adult male clean SD rats were randomly divided into three groups: sham operation group(Sham group, n = 12), sepsis group(Sepsis group, n = 12), erythropoietin intervention group(Sepsis + rhu EPO group, n = 12),the sepsis model of Sepsis group and Sepsis + rhu EPO group were made by classic CLP(cecal ligation and puncture) method, Sham group was operated only by abdominal surgery, find the location of the cecum,then put it back, suture the abdominal wall. Sepsis + rhu EPO group was given recombinant human erythropoietin 3000 IU / kg intraperitoneal injection immediately after surgery, other groups were injected with the same volume of saline.24 hours after abdominal surgery intestinal tissue specimens and serum samples were taken,the intestinal tissue was to observe pathological changes by HE, serum sample was to detect TNF-? and DAO(diamine oxidase) by Elisa,intestinal tissue homogenates was to detect MPO(myeloperoxidase)?MDA by spectrophotometer, EPOR(erythropoietin receptor) by Elisa, the expression of NF-?b was observed in the intestinal tissue by immunohistochemistry.Results Compared with Sham group, intestinal villus tissue of Sepsis group damage improved significantly, TNF-??DAO?MPO?MDA and the expression of NF-?b significantly increased,EPOR in mucosa downregulated(P <0.05); compared with Sepsis group, intestinal villus tissue of Sepsis + rhu EPO group damage decreased, TNF-??DAO?MPO?MDA and expression of NF-?b decreased, EPOR in mucosa upregulated(P <0.05).Conclusions EPO has a protective effect for intestinal tissue of sepsis rats, mainly in the inhibition of tissue peroxidation damage, inhibition of inflammatory cell activation, protect the intestinal barrier function, etc.,which may be associated with inhibition of NF-?b expression, promoting EPOR upregulation. |
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