| Schistosomiasis is one of major infectious diseases in our country.Trapped in host tissue, schistosomeegg induces granuloma response which is the main pathology of schistosomiasis. Study the process of granuloma formation and its mechanism are essential for the prevetion and treatment of this disease. Sj E16.7 is an egg specific antigen of Schsitosoma japonicum. Our perivous study showed Sj E16.7 can activate innate immune cells, but its detail mechanisms are still uncovered. In this study, we focus on the roles of Sj E16.7 on macrophage activation. The results of in vitro transwell assay showed Sj E16.7 significatly inducesmacrophage migration. The chemotactic response of macrophage to schistosome egg soluble antigencan be significantly reduced by anti-Sj E16.7 antibody. This result indicates Sj E16.7 plays important roles in schistosome egg induced macrophage migration.QPCR and ELISA results showed Sj E16.7 significantly induces macrophage cytokine production(IL-10,IL-12,IL-6,TNF-α).Moreover Sj E16.7 treatment increases MHC Class II molecules expression on the surface of macrophages.The activation of JNK and NF-κB pathwayismarkly triggered by Sj E16.7. In vivo mouse lung granuloma model showed, blocking Sj E16.7 significantly preventsgranuloma developmentvia reducingleukocyte infiltration and granuloma size. Our results suggest Sj E16.7 is one of key pathogenic factors in schistosome egg, and is a potential treatment target for this disease. |
