The Antimicrobial Activity And Quality Assessment Method Of Potentilla Chinesis Ser.

Objective To study the antibacterival activity of Potentilla chinesis Ser. samples and to establish the HPLC fingerprint, the flavonoids are the main active constitue- nts of Potentilla chinesis Ser., and to establish a method for simultaneous determination of five flavonoids. Aimed at for the scientific evaluation of Potentilla chinesis Ser. and quality control to provide the basis. And for the development and utilization of Potentilla chinesis Ser. and clinical application of provides the certain reference value.Methods Using soxhlet extraction method to extract the Potentilla chinesis Ser., the extracts from different parts were extracted by petroleum ether, chloroform, ethyl acetate and butyl alcohol in turn. To measure the minimal inhibitory concentration(MIC) by paper disk method. By high performance liquid chromatography(HPLC) to establish fingerprint of Potentilla chinesis Ser., Chromatographic conditions as follws: Agilent ZORBAX SB-C18(4.6 mm×250 mm, 5 μm) column was adopted, column mobile phase: A acetonitrile: 0.2% phosphoric acid solution system(5:95), B acetonitrile: 0.2% phosphoric acid solution system(70:30), Gradient elution, The mobile phase flow rate was 1 m L·min-1, the detection wavelength was 320 nm. The similarity were evaluated from different sources of Potentilla chinesis Ser. by Computer Aided Simlarity Evaluation software. Through established an HPLC method for simultaneous determination of five flavonoids.Results Extraction in ethyl acetate has the best antibacterial effect. The MIC is 0.0625 g·m L-1 for escherichia coli, and 0.125 g·m L-1 for staphylococcus aureus, pseudomonas aeruginosa and bacillus subtilis in the presence of this extraction. Furthermore, the common pattern of 10 samples was constructed. Different batches of the long column Hypericum fingerprints have good similarity. Established calibration Potentilla chinesis Ser. fingerprints on the basis of the area of 11 common peaks, the five compounds of identificated were rutin, hyperin, quercetin, apigenin and kaempferol. The calibration curves of five compounds showed good linearity in the ranges of concentration, the linear ranges of rutin, hyperin, quercetin, apigenin and kaempferol were 12.50-200, 6.25-100, 6.25-100, 4.00-64.00 μg·m L-1. The RSDs of precision reproducibility and stability tests were less than 2.0%, the average recoveries were in the range of 97.7%-99.4% and the range of RSDs was 1.5%-2.9%.Conclusions The Potentilla chinesis Ser.extract have good antibacterial effect for escherichia coli, staphylococcus aureus, pseudomonas aeruginosa and bacillus subtilis. The HPLC fingerprint was with good stability, precision and reproducibility, meanwhile the similarities of samples met the requirement of the fingerprint. Through establish- ed an HPLC method for simultaneous determination of five flavonoids for the first time. And can be used for the basic research and quality control of Potentilla chinesis Ser..