A Primarily Study Of The Three-dimensional Morphological Changes Of Cortical Neurons In Alzheimer’s Model Mouse

Alzheimer’s disease(AD) is neuropsychiatric disorder which is related to age. It will seriously affect the patient’s ability of learning and memory. Significant pathological features and morphology and volume changes of the neuron can be found when in the pathogenesis process of AD. Research on these changes, especially in quantitative analysis of neuronal morphology will do good to understand the mechanism of pathogeny and develop the therapeutic drugs. However, the present methods for researching on AD’s neuronal morphology are tissue sections and staining coupled with two-dimensional image analysis, which can’t analyze the intact neuronal morphology continuously because of lacking enough depth for imaging. Besides, these methods must expend plenty of time and manpower. Therefore, we use the Micro-Optical Sectioning Tomography platform and Golgi staining techniques to obtain the whole brain dataset of AD model mouse, and then, we do the three-dimensional quantitative analysis of neuronal morphology in some brain regions, such as lateral entorhinal cortex.Based on the Micro-Optical Sectioning Tomography platform and Golgi staining method, we get the whole brain dataset in high resolution of the AD model mouse in different developmental stages. Then through the three-dimensional data scanning software, we reconstruct the neurons in the brain limbic system, such as cingulate cortex and lateral entorhinal cortex. Finally, we obtain the intact three-dimensional morphology of the neurons. At the same time, MATLAB is used to do the statistic of the numbers and the length of neurons’ branches, and make the preliminary analysis. The results show that the numbers and the length of neurons’ branches degenerate gradually with the development of the disease.In order to analyze the pathomechanism of three-dimensional morphological changes of neurons, with the application of the whole brain frozen section techniques combined with immunohistochemical staining method and Nissl staining method for cytoarchitectural reference, we analyze the changes of senile plaques in relevant brain regions during the development. We image a total of about 2200 sections, then discuss the changing mechanism of three-dimensional morphology of neurons. At last, for easily sharing the results, we design a website to display the neuronal morphology.