Study On Identification Of Traditional Chinese Medicines By The Application Of Oligosaccharides From Polysaccharide Degradation Products

Identification of traditional Chinese medicines(TCMs) is to identify and discriminate TCMs effectively and accurately based on their features, especially for those confusable or relative species. There are many kinds of TCMs used through the ages, and the phenomenon such as homonym or homonym has important influence on the security and effectiveness when TCMs applicated. Origin identification, macroscopical identification and microscopical identification rely largely on experience, influenced by many factors. At present, identification mainly aims at small molecule compounds of secondary metabolites, and the main technology is chromatographic fingerprint analysis. It is difficult to achieve an effective identification for TCMs with lower contents of small molecules. Polysaccharide is a sort of characteristic ingredient in TCM, and has received more and more attention in therapy followed with its natural and secure potency, such as immunomodulatory, anti-tumor, anti-inflammatory, anti-aging and so on. Polysaccharide is generally comprised of specific repeating units, i.e., oligosaccharide segments. Depending on these fragments, polysaccharide can play many active effects. In addition, great differences had been proved in the structure of oligosaccharides from different TCM polysaccharides, and the fragments are stable after partial degradation under some conditions. The differences among the oligosaccharides only come from the single TCM polysaccharide, so the information existed in oligosaccharide segments can reveal characteristic information of TCM.Therefore, based on the principle of TCM fingerprint identification, a new identification method was established to separate TCM oligosaccharides by HPLC and used to identify confusable TCM employed the obtained fingerprints of oligosaccharides assisted with cluster analysis. It could provid basis for the quality standards and structure-activity relationship study.Chapter one: The research progress of TCM polysaccharides and oligosaccharides, polysaccharide degradation process, derivative reagents, TCM identification, and the methods of saccharides analysis were overviewed.Chapter two: A HPLC method of the separation of TCM oligosaccharides was established, and characteristic spectra were used to simultaneously identify three groups of 18 batches of confusable TCMs assisted with cluster analysis, including Glehniae Radix and Adenophorae Radix, Achyranthis Bidentatae Radix and Cyathulae Radix, Sophorae Tonkinensis Radix Et Rhizoma and Menispermi Rhizoma.Chapter three: The HPLC identification of three groups of 22 batches of TCMs from the same family and genera and the optimization of oligosaccharide degradation were performed, including chrysanthemi flos and chrysanthemi indici flos, atractylodis rhizome and atractylodis macrocephalae rhizome, angelicae sinensis radix and angelicae dahuricae radix.Chapter four: The identification of TCMs from the same family and genera by an established CZE method was performed, and the comparison of the above with HPLC identification method was also discussed.Chapter five: Preliminary identification of oligosaccharide peaks by ESI-IT and MALDI-TOF mass spectrometry was explored.Chapter six: Conclusion and expectation.