Expression And Significance Of ABCG2 And Nanog In Cervical Squamous Cancer

Objective: To discuss stem cell-related genes ABCG2 and Nanog expression in cervical squamous cancer for providing the basis for further study of cervical squamous carcinoma radiotherapy and chemotherapy tolerance mechanism. Methods: Immunohistochemistry was used to detect ABCG2 and Nanog expression in 20 cases of normal cervical, 20 cases of high grade cervical intraepithelial neoplasia, 84 cases of cervical squamous cancer, including 4 cases of highly differentiated tissue, 71 cases of moderately differentiated tissue and 9 cases of poorly differentiated tissue. In cervical squamous cancer, there were 3 cases of stage IA2, 23 cases of stage IB, 10 cases of stage IIA, 26 cases of stage IIB, 3 cases of stage IIIA, 18 cases of stage IIIB and 1 case of stage IV. All of the cervical squamous cancer patients from stage IA to stage IIA had received standard treatment, and none of them had appeared local recurrence after 2 years. Patients from stage IIB to stage IV who had received concurrent chemoradiotherapy were divided into 8 patients who had chemoradiotherapy tolerance, and 40 patients who were sensitive to chemoradiotherapy after 2 years. All specimens were from cancer before surgery or chemoradiotherapy. All immunohistochemical images were treated by IPP6.0, and calculated integral optical density(IOD). Then SPSS 22.0 software was used to analyze them for exploring the role of ABCG2, Nanog expression in cervical squamous cancer development and relationship between cervical cancer grading and chemoradiotherapy resistance. Results:(1) ABCG2, Nanog expression were obviously increased with increasing cervical lesions in normal cervical tissue, high-grade cervical intraepithelial neoplasia tissue, cervical squamous cancer tissue(P<0.05).(2) ABCG2, Nanog expression were obviously reduced with increasing degree of differentiation in poorly differentiated, moderately differentiated, well-differentiated cervical squamous cancer tissue(P <0.05).(3) ABCG2, Nanog expression in cervical squamous cancer which tolerated chemoradiotherapy were significantly higher than chemoradiotherapy-sensitive cervical squamous cancer(P <0.05). Conclusion: ABCG2, Nanog expression not only may be related to the development and differentiation of cervical squamous cancer, but also may be related to cervical squamous cancer chemoradiotherapy resistance.