A Case-Control Study On The Relationships Among Noncoding RNA,Environmental Factors And Hypertension

Objective A case-control study was conducted to investigate major risk factors of hypertension and analyze the differential expression of noncoding RNA?long noncoding RNA and mi RNA? in hypertensive patients and control groups. Controlling environmental risk factors, whether genetic factors were associated with the prevalence of hypertension? To investigate the regulatory role of noncoding RNA in the occurrence and development of hypertension, to further understand the molecular genetic mechanism of hypertension. It may provide scientific basis for the risk assessment and management of hypertension and cardiovascular diseases. Method A total of 328 essential hypertension patients and 328 healthy volunteers without any evidence of essential hypertension or inflammatory disorders were assessed from November 2014 to December 2015 at the Fujian Medical University Union Hospital and the First Affiliated Hospital of Fujian Medical University, China. General exclusion criteria were a known history of leukopenia, thrombocytopenia, or severe hepatic or renal dysfunction, as well as evidence of an inflammatory or malignant disease. All patients were fully informed of the study procedures before they provided consent. Questionnaire investigations, physical examination and laboratory detection were conducted. Quantitative real-time PCR was used to profile the expression levels of the following lncRNA and miRNA previously demonstrated to be associated with essential hypertension: NR₀27032, NR₀34083, NR₁04181, miR-126, miR-143 and miR-145. Multiple factor logistic regression was used to analyze the influence of environmental factors and genetic factors on the prevalence of hypertension. Result?1? Multivariate logistic regression analysis indicated that A type of personality, anxiety, obesity, abdominal obesity, and hypertension family history were risk factors for hypertension, while light diet and physical activity were protective factors.?2? The expression of NR₀27032 and NR₀34083 were lower in the case group than that in the control group, while NR₁04181 in the case group was higher than that in the control group.?3? After controlling other risk factors, multivariate logistic regression analysis showed that higher expression levels of NR₁04181 and the lower expression levels of NR₀27032 and NR₀34083 were independent risk factors for hypertension.?4? In case group, miR-143 and miR-145 were higher than the control group, the expression of miR-126 was not statistically significant difference in the case group and the control group.?5? After controlling other risk factors, multivariate logistic regression results suggests that higher expression level of miR-143 was an independent risk factor of hypertension. Conclusion?1? The occurrence and development of hypertension is the result of comprehensive effect of multiple risk factors, psychological and social factors?A type of personality and anxiety?, physiological index?obesity and abdominal obesity? and family history of cardiovascular?family history of hypertension? were hypertension risk factors, while the behavior of the individual factors?light diet and appropriate physical activity? were protective factors. In order to prevent hypertension's occurrence and development effectively, we should according to the social-environment- psychological-biological medical model to management and guidance the risk factors of hypertension.?2? After controlling other risk factors, higher expression levels of NR₁04181 and miR-143, as well as lower expression level of NR₀27032 and NR₀34083 were independent risk factors of hypertension, which suggested that the noncoding RNA may play an important role in the occurrence and development of hypertension, pending further study to explore the regulation mechanism of noncoding RNA on blood pressure.