Effects Of Resveratrol On The Migration Of Human Retinal Pigment Epithelial Cells

Aim To investigate the effect of resveratrol(Res) on human retinal pigment epithelial cell(ARPE-19) migration, to explore the possible molecular mechanisms, and to supply theoretical bases for the research of the treatment of proliferative vitreoretinopathy.Methods ARPE-19 cells were treated with Res at different concentrations of 0, 50, 100, 150, 200 and 300?mol/L for 24 h, 48 h and 72 h, with 0?mol/L as the control group, and Cell Counting Kit-8(CCK-8) test were performed to detect cell proliferation, and a safe and effective range of concentration was selected for subsequent tests. 0, 100 and 200?mol/L of Res were chosen. Wound-healing assay was conducted when ARPE-19 cells in a scratch wound model were treated using Res for 24 h, and the wound-healing extent was observed. Transwell migration assay was carried out to testify cell migration situation when cells in Transwell chambers were disposed by Res for 16 h and 24 h, and the cell migration ratio was calculated; Western blot was carried out to identify the expression of matrix metalloproteinase-9(MMP-9) and p38 mitogen-activated protein kinases(p38 MAPK), when cells were disposed by Res for 24 h.Results CCK-8 revealed that Cell proliferation was inhibited by Res at a time and dose-dependent manner, and compared with the control group, the cell proliferation index was decreased to about half when cells were pre-treated with Res(100~200?mol/L; for 48h). Wound-healing assay showed that compared with the control group, the wound-healing extent was decreased when cells were pre-treated with Res(100?mol/L and 200?mol/L; for 24h). Transwell migration assay showed that when cells were pre-treated with 100?mol/L and 200?mol/L Res for 24 h, cell migration ratios were(0.6479±0.075)% and(0.4313±0.040)% respectively, and when pre-treated for 16 h, cell migration ratios were(0.6049±0.076)% and(0.3598±0.062)% respectively. Western blot displayed that the expressions of MMP-9 and p38 MAPK were down-regulated when cells were pre-treated with Res(100?mol/L and 200?mol/L; for 24h).Conclusion Res could inhibit APRE-19 cell migration, and the potential mechanisms may involve with the down-regulation of the expression of MMP-9 and p38 MAPK.