The Inhibition Of Resveratrol In Combination With Ara-C On Human Leukemia K562 Cell

OBJECTIVES: Resveratrol is a natural plant extraction with much bioactive function, and it also has antineoplastic effect to many kinds of tumours without toxic effect, so it is prospected to be a newly valuable pharmaceutical product to prevent and treat human cancers. To investigate synergistic effect of resveratrol and Ara-c and discuss its related mechansisms by observing suppressing effect, cell cycle, protein expression of Fas/FasL and Survivin when K562 cell was treated with resveratrol alone or in combination with Ara-c.Method: MTT assay was performed to detect the suppressing effect of resveratrol and Ara-c alone or in combination on K562 cells for 24h, 48h and 72h. Chou-Talalay was used to calculate middle dosage at different periods to estimate synergistic effect of resveratrol and Ara-c on K562 cells. After K562 cells were treated by resveratrol and Ara-c alone or in combination for 48h, flow cytometry (FCM) was performed to observe cell cycle distribution; transmission electron microscopy was performed to observe the apoptosis body; immunocytochemical staining was performed...