Study Of Gambogenic Acid On Apoptosis Of Multiple Myeloma Cells

Objective:Base on exploring the effect and mechanisms of apoptosis of multiple myeloma MM.1S cells induced by gambogenic acid (GNA), our aim is to provide laboratorial evidence for screening anti-tumor agents and developing more effective treatment of multiple myeloma.Methods:1. MM.1S cells were interfered by different concentrations of GNA for 24h,48h and 72h, respectivley. The morphologies of the cells were observed through the microscope;2. The growth inhibition of MM.1S cells induced by GNA for 24h,48h and 72h were detected by CCK-8 assay;3. Cell cycle and apoptosis of MM.1S cells were tested by flow cytometry (FCM);4. Expressions of apoptosis-related proteins Cleaved PARP, P53, Cleaved Caspase-3, Bax and Bcl-2 after treatment by different concentrations of GNA were detected by Western blot.Results:1. After treatment by different concentrations of GNA, the volume of part of MM.1S cells became smaller than that of normal cells, and some cells had irregular shapes.2. After treatment by different concentrations of GNA for 24h,48h and 72h, the inhibition of MM.1S cells showed a time-and concentration dependence. IC50 of GNA at 24h,48h and 72h were about 1.299?M,0.883?M,0.752?M, respectively.3. MM.1S cells were arrested at G2/M phase induced by GNA. Compared with that of the control group, the apoptosis rate of MM.1S cells were obviously increased after GNA therapy.4. Expressions of Cleaved PARP, P53, Cleaved Caspase-3 and Bax of MM.1S cells were increased significantly and expression of Bcl-2 was decreased significantly after therapy of GNA compared with that of the control group.Conclusions:GNA has obvious effect on the inhibition of growth, cell cycle arrest and apoptosis of MM.1S cells. GNA at high concentration could significantly induce apoptosis of MM.1S cells. The anti-multiple myeloma effect of GNA might be through the expression of the apoptosis-related proteins such as PARP, P53, Caspase-3, Bax and Bc1-2.