0bservation Of Impact That The Huoxue Pingtu Powder Has On Retrobulbar Fibroblasts After Stimulated By IFN-γ

Objective:1) Huoxue san observed sudden containing serum levels of IFN-γ after stimulation of fibroblast proliferation balls later,HA, Ⅰ collagen,Ⅲ collage synthesis, MMP-1,MMP-2,TIMP-1,TIMP-2activity,CD40,CD54,HLA-DR expression;2)To investigate the mechanism Huoxue flat suddenly scattered TAO treatment.Methods:After the normal sources ball fibroblasts in vitro primary,subculture,take 2-8 generations of cells were identified,defined as the ball fibroblasts grouping experiments.The control group continued to train with normal culture medium;blank control group of rats serum medium containing 20%normal rat serum and cultured;blank stimulation with 200u/medium ml IFN-γ containing cultured;Huoxue flat San sudden intervention group with 20% serum-containing Huoxue level suddenly scattered and medium 200u/ml IFN-γ in the drug continued to train.Then using MTT assay,ELISA method,immunohistochemistry,flow cytometry proliferation RFs of each group,HA, Ⅰ collagen,Ⅲcollagen synthesis,MMP-1,MMP-2,TIMP-1,TIMP-2 activity, CD40,CD54,HLA-DR expression in the case,and the experimental data for statistical analysis.Results:1)MTT assay OD values of the four groups to assess the proliferation of RFs in each group,the control group showed no significant difference(P>0.05)in rat serum blank control groups;blank stimulus OD values than the control group,serum blank control group of rats was increased,the difference was statistically significant(P<0.05);Huoxue flat casual sudden intervention group OD values were significantly lower than the blank stimulus,and the difference was statistically significant(P<0.05).2))detected by ELISA four groups HA, Ⅰ collagen synthesis of collagen typeⅢblank stimulation HA, Ⅰ collagen,Ⅲcollagen synthesis than the control group,serum blank control group of rats was increased,and the difference statistically significant(P<0.05);Huoxue flat casual sudden intervention group HA, Ⅰ collagen,Ⅲcollagen synthesis stimulation group was significantly lower than in the blank,and the difference was statistically significant(P<0.05).3)detected by immunohistochemistry four groups of MMP-1MMP-2 activity,the control group showed no significant difference(P>0.05)in rat serum blank control groups;blank stimulation MMP-1,MMP 2 activity than the control group,serum blank control group of rats was lower,the difference was statistically significant (P<0.05);Huoxue flat casual sudden intervention group MMP-1,MMP-2 activity was significantly higher than the control sti mul ati on, the difference was statistically significant(P<0.05).4)detected by immunohistochemistry four groups of TIMP-1,TIMP-2 activity,the control group showed no significant difference(P>0.05)in serum blank control group,two groups of rats;blank stimulation TIMP-1,TIMP 2 activity than the control group,serum blank control group of rats was increased,the difference was statistically significant(P<0.05);Huoxue flat casual sudden intervention group TIMP-1,TIMP-2 activity was significantly lower than the control stimulus,the difference was statistically significant(P<0.05).5)by flow cytometry fourCD40,CD54,HLA-DR expression,blank control group,no significant difference(P>0.05)in rat serum blank control groups;blank stimulation CD40,CD54,HLA-DR expression compared with the control group,serum blank control group of rats was increased,the difference was statistically significant(P<0.05);Huoxue flat casual sudden intervention group CD40,CD54,HLA-DR expression stimulation group was significantly lower than in the blank,and the difference was statistically significant (P<0.05).Conclusion:Huoxue flat suddenly scattered through ball after inhibition of fibroblast proliferation,inhibition of CD40,CD54,HLA-DR expression,regulate MMPs/TI-MPs balance,inhibit HA I collagen synthesis,Ⅲcollagen to treat TAO.