GS,AC,PKA And RyR2 Protein Expression Levels And Coronary Heart Heart Disease Qi Deficiency Syndrome Correlation Of Incidence

Objective:To study coronary heart disease(coronary artery heart disease, CHD) heart qi deficiency microscopic mechanisms of pathogenesis of Coronary Heart Disease Qi Deficiency Syndrome theory summarized and outlined in GS(GTP-binding protein, GTP binding protein), AC(adenylate cyclase, adenylate cyclase), PKA(cAMP-dependent protein kinase a, protein kinase a), RyR2(ryanodine receptor, ryanodine receptor) protein expression levels and the incidence of Coronary heart Disease Qi Deficiency Syndrome correlation as the starting point Discussion Coronary heart Disease Qi Deficiency Syndrome possible pathogenesis.Methods:The "left coronary artery ligation plus vasopressors L- NNA" Preparation of coronary heart disease rat model of Qi Deficiency Syndrome, and the rats were randomly divided into control group, model group, sham group, Yiqi Huoxue Fang group(high,medium, low), the WM group(diltiazem hydrochloride). In 45 days after modeling under anesthesia, take myocardium, Western blot assay GS, AC, PKA, RyR2 protein expression changes at the protein level.Results:In general, changes in rats after modeling and clinical characteristics of patients with Coronary Heart Disease Qi Deficiency Syndrome in line, GS, AC, PKA,RyR2 protein in myocardial tissue of rats in each group and internal reference GAPDH gray gradation ratio difference(P <0.05), which compared with the model groups increased significantly(P <0.01); compared with the model group reduces high dose group was significantly(P <0.01).Conclusion:The "left coronary artery ligation plus vasopressors L- NNA" Preparation of coronary heart disease rat model of heart qi deficiency disorders is an ideal combination of modeling methods. The results showed that GS, AC, PKA protein level and Coronary Heart Disease Heart Qi Deficiency Syndrome correlation, RyR2 openGS-AC-cAMP-PKA signaling pathway mediated exception is likely to be the main mechanism for the onset of coronary heart qi deficiency.