Effct Of Low Expression Of PRMT2 On The Autophagy Induction Of MCF7 Cells And Its Mechanism

Objective: To investigate the effect of low expression of PRMT2 on the autophagy induction of human breast cancer MCF-7 cell and its potential mechanism.Methods: According to the expression level of PRMT2 in human breast cancer MCF-7 cell, the experiment was divided into two groups:normal MCF-7 group and PRMT2-miRNA-MCF-7 group. At first, the relationship between PRMT2 and autophagy was discussed in human breast cancer MCF-7 cell through Beclin1 and LC3 protein determination.The protein level was detected by Western Blot. Next, the molecular mechanism of that PRMT2 inhibited autophagy was preliminarily explored by detecting autophagy signal pathway protein(mTOR; ULK1;p-AMPK; AMPK). The protein level was also detected by Western Blot.Thirdly, the potential signal pathway was investigated by detecting the interrelation, which was detected by co-immunoprecipitation, of PRMT2 and pathway protein in MCF-7 cell.Results: 1 Western blot detectations reveal that the expression of Beclin1 and LC3 increase in PRMT2-mi RNA-MCF-7 cell. 2 The expression of AMPK were undifferentiated between the two groups,while the expression of ULK1, mTOR, p-AMPK increased in PRMT2-mi RNA-MCF-7 cell. 3 The co-immunoprecipitation detectations reveal PRMT2 could interact with AMPK, and Beclin1 could interact with PRMT2 and AMPK.Conclusion:PRMT2 might interaction with AMPK and effect autophagy induction through AMPK/ULK1/Beclin1 signaling pathway in human breast cancer MCF-7 cell.