Emperimental Study To The Impaction Of Silver Ions To Bacteria In The Silicone Tube Surface

Tuberculous hydrocephalus is the most common complication of tuberculous meningitis, Ventricle Peritoneal Shunt which is given to cure it first, as for invalided patients by internal medicine conservative treatment, has been the main treatment of tuberculous hydrocephalus. The most common complications is infection, and congestion, especially in patients with tuberculous hydrocephalus, whose infection is uncontrol. Shunt tubes as a foreign body device are implanted into the body, and will most likely cause of mycobacterium tuberculosis and other bacterial's adhesion, lead to infections of the biological materials, which has has become the most major problem of implant materials' clinical application effect. We know about the nano-silver material has good antibacterial ability, and wide antimicrobial spectrum, and strong antimicrobial properties, and without the advantages of resistance, is a kind of natural antibacterial material, and the study of mycobacterium tuberculosis is still lack. The antibacterial mechanism of silver materials includes damaging bacterial DNA, interrupting cell signal transduction, damaging active oxygen free radical, and bacterial protein interaction, and cell wall peptidoglycan and plasma membrane function. We conclude that nano-silver material can restrain the growth of bacteria and reduce the bacteria adhesion on the surface of silica gel tube, which can reduce the chance of infection, and congestion.We choose the BCG vaccine and Staphylococcus aureus to simulate process of infection, and make the shunt pipe surface of sulfadiazine silver coating as experimental group, uncoated shunt tube as a comparison, shunt by staphylococcus aureus and BCG, according to the division cycle and culture period sampling. Compare antibacterial ability according tothe same algebraic colony evaluation shunt.Growth curve drawing: in Staphylococcus aureus bacteria liquid every 1 h we take bacterium fluid, in the BCG bacteria liquid every 3 d we take bacteria liquid, by measuring the OD value of bacteria liquid, and draw the growth curve in ordinary silicone tube and silver-plated silicone tube group.Adhesion curve drawing: in staphylococcus aureus bacteria liquid every 2.5 h in the silicone tube, the BCG vaccine in bacteria liquid every five days take silicone tube, by elution, transfering, solid medium culturing, respectively to observe two kinds of silicone tube in solid medium culture to compare the number of colonies, and draw adhesion curve.Electron microscope: choose the silicone tube in BCG bacteria liquid after 30 days and in staphylococcus aureus bacteria liquid after 15 hours, fixation, dehydration, drying, electron microscope, taking meaningful visions for statistical analysis.According to growth curve we can see the OD value of BCG and staphylococcus aureus' s bacteria liquid in silver-plated silicone tube group is lower than those of common silicone tube group, the difference is statistically significant(P < 0.05); According to adhesion curve we can see bacteria colonies in silver-plated silicone tube group by the BCG and staphylococcus aureus are relatively less common silicone tube group, the difference is statistically significant(P < 0.05). Electron microscope showed the number of the BCG vaccine in silver-plated silicone tube is 0.7±0.67495, in the common silicone tube group the number is 3.7±1.15950, the difference is statistically significant(P < 0.05); the number of Staphylococcus aureus in silver-plated silicone tube is 21.5±5.99, the common silicone tube is 605.33±96.43, the difference is statistically significant(P < 0.05).To sum up, silver ions can inhibit the growth of staphylococcus aureus and BCG, and can reduce adhesion on the surface of the silicone tube.