| Background: Transforming growth factor-beta1 (TGF-β1) has been implicated in the inflammatory response and subsequent scarring during wound healing. Objective: The experiment was designed to evaluate the effects of a topical application of exogenous TGF-βantibody on the neural regeneration. Methods: Seventy-two masculine SD rats were divided into four groups at random. Twenty-four rats were composed of the contrast control with 30μl isotonic Na chloride inlet into stoma, the experimental effects of which was compared with another twenty-four rats (the experimental control) with 30μl TGF-βantibody inlet into stoma. Twelve rats were composed of the normal control with 30μl Na chloride inlet into infra-epineurium without the nerve being disconnected, the experimental effects of which was compared with another twelve rats with 30μl TGF-βantibody inlet into infra-epineurium without the nerve being disconnected. Each group was detected through general observation, electrophysiologic study, ultrastructural organization and pathematology dyeing detection. Results: The results of the neural electrophysiologic study after twelve weeks show that the neural regeneration situation of the experimental control was superior to the contrast control in nerve fiber specific staining, myelin staining, MESSION staining and electron microscope, etc. . And so were the results of MESSION staining and electron microscope, etc. after four weeks. Conclusion: TGF-βantibody obviously promotes rats'neural regeneration. |
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