The Effects Of Adding And Subtracting Radix Achyranthis Bidentatae On The Effective Components Of Tongsaimai In Vitro And In Vivo

Tongsaimai pellets(excluding radix achyranthis bidentatae)for the treatment of ischemic stroke is secondary development based on the famous traditional Chinese medicine Tongsaimai Tablet(contain radix achyranthis bidentatae)for the treatment of thrombosis obliterans.Tongsaimai pellets is composed of six herbals including Flos Lonicera japonica,Radix Astragali,Radix Angelica sinensis,Radix et Rhizoma Glycyrrhizae,Radix Scrophulariae.Tongsaimai pellets is better than Tongsaimai Tablet on the treatment of ischemic stroke the primary efficacy index.The study put forward the medicinal properties of characteristics because of the"down" of radix achyranthis bidentatae,choose radix achyranthis bidentatae may lead to drug major active constituent parts quality and quantity distribution change on the base of the treatment ischaemic constituent parts.With the research of adding and subtracting radix achyranthis bidentatae for the change of material group inside and outside on the base of Tongsaimai pellets revealing the medicinal properties of characteristics "down".Lay the foundation for the subsequent further study of the medicinal properties of the material basis,of the body process and mechanism of action of radix achyranthis bidentatae,but also provide a basis for the reasonable of the removal of radix achyranthis bidentatae on the treatment of ischemic stroke with Tongsaimai.Use UPLC established the UPLC-UV method of Tongsaimai chemical samples and tissue samples,and joint MS-Q-TOF technology preliminary analysis the chemical composition of Tongsaimai adding and subtracting radix achyranthis bidentatae chemical sample and each tissue sample.The study comprises three parts:The first part is the studies on adding and subtracting radix achyranthis bidentatae to the effect of Tongsaimai groups of substances in vitro.Tongsaimai subtracting radix achyranthis bidentatae analyses the 31 possible compounds.Tongsaimai adding radix achyranthis bidentatae analyses the 35 possible compounds.To radix achyranthis bidentatae itself analyses the 12 possible compounds.Tongsaimai adding radix achyranthis bidentatae have some compounds comes from radix achyranthis bidentatae,it is not too big effect for the other active ingredient species in Tongsaimai pel lets.Adding and subtracting radix achyranthis bidentatae have some effect on the main active ingredient of Tongsaimai in the chemical sample from the analysis of chemical sample.Also established the UPLC double wave method to assay the content of Chlorogenic acid and Ferulic acid,Rutin,Harpagoside,Liquiritin,Liquiritigenin,4,5-diCQA for the Tongsaimai middle extract.Methodoloogy result is good,can be used as reliable means of evaluation and analysis of the quality for Tongsaimai intermediate extract.So as to provides the foundation for adding and subtracting radix achyranthis bidentatae to the effect of Tongsaimai groups of substances in vivo.The second part is the studies on adding and subtracting radix achyranthis bidentatae to the effect of Tongsaimai groups of substances in vivo.Through comparing the differences among the different groups of tissue samples obtained the effect of Tongsaimai groups of substances in vivo from radix achyranthis bidentatae.Experimental mice as experimental animals,Reference to the suture method to copy the middle cerebral artery ischemia modal preparing the tissue samples after the mice are dosed by irrigating stomach.Preliminary analysis each organization samples,we found the 7 prototype components through the UPLC/MS-Q-TOF technology.And find the differences of the prototype components from Tongsaimai in distribution among the various organizations.Guess the target site where the active ingredient from Tongsaimai works in the vivo.Suggesting the affect that radix achyranthis bidentatae to the main active ingredient in Tongsaimai combined with groups of substances in vitro.The third part is the summary of the full text,research the distribution in vivo of the active ingredient that work for the treatment of ischemic stroke in tongsaimai on the basis of the effect that radix achyranthis bidentatae to Tongsaimai groups of substances in vivo and in vitro.Through testing the active ingredient content within the organization to illustrate the medicinal properties of characteristics of radix achyranthis bidentatae "down".Laid the basis of medicinal the base on substances,process in the vivo and mechanism of action for follow-up study of radix achyranthis bidentatae.