| Objective:This study through independent rat drinking furazolidone aqueous solution(press 1 kg of water to add 700 mg of furazolidone configuration),DCM rats model,to observe the Kang xian yi xin nong suo wan on DCM rats serum BNP,TNF-? and myocardial tissue Ang?,explore its mechanism of action of prevention and treatment of dilated cardiomyopathy,to provide scientific experimental basis for the clinical application in the future.Methods:1 The selection of weight 60-70 g,SPF health male Wistar rats.By random method according to the 7:1 first divided into model group and normal control group,to build module adopts independent drinking furazolidone aqueous solution,change daily,DCM rats model was established.2 Determination of building 8 weeks rats left ventricular ejection fraction,LVEF value 45% or less of the rat model is built by a success.Copies made mould successful rats,were randomly divided into model group,Kang xian yi xin nong suo wan high,medium and low dose group,captopril group,Chinese and western medicine combined group(Kang xian yi xin nong suo wan high-dose group and captopril),adopts the corresponding drug lavage treatment,model group daily lavage and the treatment group the same volume of physiological saline.Normal control group of conventional breeding animals,not drug intervention;Treatment group continue to own drinking furazolidone aqueous solution to ensure the continued of pathogenic factors,drug intervention in eight weeks,rat body weight,3 times a week and observation records its general state changes.3 8 weeks after drug intervention,echocardiography,for each group based in rats,enzyme linked immunosorbent test(ELISA)in rats was detected in the serum BNP,TNF-? and myocardial tissue Ang ? level,HE method to detect myocardial tissue morphology in rats and statistical analysis.Results:1 The general condition of rats,after 1 month of building,compared with the blank group,model group rats gradually appear the symptom of myocardial damage,characterized by depression,less activity,nuzzles;The fur is loose,hair colour and lustre is poor,some rats appear patchy hair removal,skin ulcer,etc.Compared with model group,the drug treatment group the symptoms later,also light.2 Echocardiography to detect cardiac function in rats: compared with normal control group,model group significantly reduced LVEF of rats(P < 0.01).Compared with model group,Kang xian yi xin nong suo wan LVEF of low dose group of rats no significant statistical difference(P > 0.05),Kang xian yi xin nong suo wan high dose group,captopril group,and combination of Chinese and western group rats LVEF values are statistically significant differences(P < 0.05);Compared with Kang xian yi xin nong suo wan low-dose group,Kang xian yi xin nong suo wan high,medium dose group,captopril group and combination of Chinese and western LVEF value of rats were significantly higher(P < 0.05).3 In the rat serum BNP level: Model group,expression level of serum BNP obviously increased,the result of different things has significant statistical significance(P< 0.01);And compared with tings and model group,the serum BNP Kang xian yi xin nong suo wan low-dose group expression level is lower than the model group,but there was no statistically significant difference(P > 0.05),captopril group,Kang xian yi xin nong suo wan high,medium dose group,the joint Chinese and western medicine group,the serum BNP level decreased significantly(P < 0.05);Compared with Kang xian yi xin nong suo wan low-dose group,Kang xian yi xin nong suo wan,medium dose group of serum BNP levels(P < 0.05).4 The rat serum TNF-?level: The exerceing tings compared with normal control group,model group,expression level of serum TNF-? increased significantly,with significant difference of statistical significance(P < 0.01);Compared with model group,Kang xian yi xin nong suo wan low-dose group,serum TNF-?expression level is lower than the model group,but the repouter we can it that there was no statistically significant difference(P > 0.05),captopril group,Kang xian yi xin nong suo wan high,medium dose group,the joint Chinese and western medicine group,the serum from TNF-? level decreased significantly(P < 0.05);Compared with Kang xian yi xin nong suo wan low-dose group,Kang xian yi xin nong suo wan high,medium dose group of serum TNF-?level lower(P < 0.05).5 Myocardial tissue Ang? level: Compared with normal control group,model group in the myocardial tissue Ang? level increased significantly,with significant difference of statistical significance(P < 0.01);Compared with model group,in addition to the Kang xian yi xin nong suo wan low-dose group,more than drug group in myocardial tissue Ang? levels have different degree of decline,and the difference was statistically significant(P < 0.05).Conclusion:1 Kang xian yi xin nong suo wan can reduced left ventricular end systolic diameter and end-diastolic diameter,raise LVEF DCM heart failure rats,improve heart function.2 Kang xian yi xin nong suo wan can reduce the DCM rats serum BNP,TNF alpha and myocardial Ang ? neuroendocrine factor expression levels,shows that fiber resistance good heart concentrated pill can inhibit the excessive activation of neuroendocrine system,and increase with the increase of the dose.3 Kang xian yi xin nong suo wan combined Western medicine in the treatment of dilated cardiomyopathy,curative effect is better,better than single western medicine. |
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