Clinical Re-evaluation Of Anchang Yuyang Decoction In The Treatment Of Ulcerative Colitis And The Effects Of TFF3, MUC2 And TLR4 Gene Expression In Rat Colon

Objective: Our previous study found that Anchang Yuyang Decoction has better treatment of ulcerative colitis and to explore its mechanism from the cytokine IL-6, IL-13 and so on. In this paper, based on previous studies, the efficacy of TCM symptoms, TCM symptom score, etc. to evaluate the clinical efficacy again and on ulcerative colitis in rats MUC2, TFF3, TLR4 gene expression in intestinal An experimental study observed Yuyang Tang Discussion Anchang Yuyang Decoction treatment of ulcerative colitis mucosal barrier repair mechanism.Methods: The clinical observation: Select Persistent active ulcerative colitis in patients with chronic or relapsing met the inclusion criteria for a total of 66 patients were randomly divided into two groups of Chinese security intestine Yuyang Tang group of 33 patients in the control group mesalazine 33 patients were taking two months, the comprehensive efficacy between the two groups, TCM syndrome, symptom score, the endoscopy of the colon mucosa efficacy, mucosal pathological effect, C- reactive protein, erythrocyte sedimentation rate, CD4 +, CD8 +, CD4 + / CD8 + of the changes, and to monitor drug safety.Animal experiment: 90 Wistar rats were randomly divided into control group, model control group, Anchang Yuyang Decoction low, medium and high dose mesalazine groups. In addition to the control group, the other groups have been applied, 2,4,6-trinitrobenzene sulfonic acid(TNBS) rat model UC, three days after the successful model, each group survived rats administered with corresponding drugs Ann intestinal Yuyang Tang low, medium and high dose groups were given medicine containing crude drug 0.75 g / m1,1.5g / m1,3g / m1 concoction gavage; mesalazine group with mesalamine suspension(0.035 g / ml) orally; blank control group, model control group are distilled water; animals in each group were fed each 2ml, 2 times a day, once every 21 days. 21 d after the rats were sacrificed, samples taken fresh colon, the mucosa of rats in each group was observed gross morphological and histological changes, histopathological scores of colonic mucosa, using RT-PCR method to detect colon tissue TFF3 MUC2, TLR4 expression.Results: In terms of a comprehensive clinical efficacy, TCM syndrome, colonoscopy mucosal efficacy, the efficacy of mucosal pathology in both groups improved to varying degrees, the experimental group and the control group, the difference was not statistically significant(P> 0.05);TCM symptoms efficacy in experimental group than the control group(P <0.05); two groups were performed before and after their treatment of TCM symptom score compared with experimental and control groups in terms of TCM symptom score improved than before treatment, the presence of statistics Learn the difference(P <0.01 or P <0.05); two groups before and after treatment, ESR, CRP, CD4 +, CD8 +, CD4 + / CD8 + improve their varying degrees, there is a significant difference(P <0.01 or P <0.05); compare the results between the two groups after the treatment, no statistically significant difference(P> 0.05).Experimental part: Compared with model group, Anchang Yuyang Decoction, the high-dose group mesalazine group histopathological score, TLR4 expression were significantly decreased(P <0.05), TFF3, MUC2 expression was significantly increased(P < 0.05). Compared with Ann intestinal Yuyang soup dose group, An intestinal Yuyang Decoction high dose mesalazine group histopathological scores were significantly reduced(P <0.05), significantly increased TFF3 expression(P <0.01). Compared with Ann intestinal Yuyang soup dose mesalazine group, significantly increased MUC2 expression An intestinal Yuyang Decoction high dose group(P <0.01), significantly decreased TLR4 expression(P <0.01).Conclusion: Anchang Yuyang Decoction has a good role in treatment of ulcerative colitis, and preliminary studies Anchang Yuyang Decoction can improve the efficacy of TCM symptoms consistent outcomes, symptom score, endoscopic mucosal effect, pathological curative effect. Its repair mucosal barrier promote ulcer healing mechanism may increase mucosal protective factor TFF3 and MUC2 gene expression and reduced inflammation mediator TLR4 gene expression.