Predictive Role Of CTCs In Liver Metastasis Of Colorectal Cancer

Objective:Testing different grouping of colorectal cancer patients the levels of circulating tumor cells(CTCs), analyze its the relationship between clinical pathologic features and liver metastasis in colorectal cancer.Methods: The peripheral blood samples were collected from 56 CRC and 15 healthy individuals.The selected cases according to the preoperative examination(conventional ultrasound, CT, MRI, etc) and operative findings are divided into: 10 cases with liver metastasis group(group A), without liver metastases group according to the postoperative follow-up of 12 months is divided into two groups: 35 cases without liver metastases group(group B), 11 cases liver metastases group(group C).First, the combination density gradient centrifugation method and positive immunomagnetic bead method was used to enrich CTCs Then,the expressions of cytokeratin-20(CK-20) and carcinoembryonic antigen(CEA) mRNA were detected by quantitative real-time reverse transcription-polymerise chain reaction(qRT-PCR).Results: 1.Group A and group B, group C, group D of CTCs positive detection rate is 80.0%, 37.1%, 81.8% and 0.0% respectively, according to a'= 0.0083 inspection level, group A, group B and group C of CTCs positive detection rate were higher than in group D, statistically significant difference(P < 0.0083).2. According to a'= 0.0125 inspection level, the positive detection rate of CTCs is higher than group B, group a statistically significant difference(P < 0.0125);the positive detection rate of CTCs is higher than group B, group C differences statistically significant(P < 0.0125);Cannot believe in group A and group C is different the positive detection rate of CTCs, no statistically significant difference(P > 0.0125).3. Patients with colorectal cancer patients with positive detection rate of CTCs with age, sex, tumor location, no correlation(P > 0.05), but with TNM staging of the tumor and the presence of lymph node metastasis.Conclusions: 1. The expression of CTCS in patients with colorectal cancer without correlated with patients' age, sex, tumor location, but the expression of CTCS significantly correlated with TNM stages and lymph node metastasis.2. Positive detection rate of CTCs in liver metastases group was obviously higher than that of without liver metastases group, prompt CTCs can be used as early predictors of colorectal liver metastases.