Curcuma Oil Injection On SKOV-3/DDP Carboplatin Resistance Reversal Effect Study

Objective:Explore non-cytotoxic concentration(IC5) of Curcuma oil injection collaborative carboplatin cisplatin-resistant human ovarian carcinoma cell lines(SKOV-3 / DDP) and carboplatin resistance reversal effect.Methods:Cell counting method to draw human ovarian cancer cell lines sensitive(SKOV-3) cisplatin-resistant cell lines(SKOV-3 / DDP) and its growth curve.With a concentration of 10 ug / ml, 20 ug / ml, 40 ug / ml, Curcuma oil injection effect 80 ug / ml, 160 ug / ml in SKOV-3 and SKOV-3 / DDP, screening Curcuma oil injection combined with concentration.With half inhibitory concentration(IC50) of Curcuma oil injection were acting on SKOV-3 and SKOV-3 / DDP 24,48,72 hour screening experiments Curcuma oil injection optimum effect long observation time to determine the optimal node.With a concentration of 15 ug / ml, 30 ug / ml, 60 ug / ml, 120 ug / ml, carboplatin 240 ug / ml in SKOV-3, derived its IC50; then the concentrations of 60 ug / ml, 120 ug / ml, 240 ug / ml, 480 ug / ml, carboplatin 960 ug / ml in SKOV-3 / DDP, draw their IC50; calculation of carboplatin resistant index of SKOV-3 / DDP's(RF).Setting the blank negative control group, IC50 carboplatin positive control group, non-cytotoxic concentration(IC5) Curcuma oil injection group, IC5 Curcuma oil injection and IC50 carboplatin group, using tetrazolium blue(MTT) colorimetry, select the 570 nm wavelength, ELISA each well was measured absorbance(OD) compared Curcuma oil injection collaborative carboplatin SKOV-3 / DDP proliferation inhibition.Set different concentrations of carboplatin group and the corresponding concentration of carboplatin and IC5 Curcuma oil injection combination group, using the MTT assay, is able to change carboplatin SKOV-3 / DDP proliferation comparative associated with IC5 Curcuma oil injection after inhibition rate was calculated Curcuma oil injection of carboplatin resistant reversal index(RI), described IC5 Curcuma oil injection on SKOV-3 / DDP carboplatin resistance reversal situation.Results:Sensitive human ovarian cancer cell lines(SKOV-3) and its resistant cell lines(SKOV-3 / DDP) growth curves showed in vitro growth of SKOV-3 / DDP than SKOV-3 slow; according to both the growth in vitro, to determine experimental select Training day 2 cells.Curcuma oil injection on SKOV-3 and SKOV-3 / DDP inhibited the proliferation showed a concentration-dependent, inter-dose showed a positive correlation; Curcuma oil injection on SKOV-3 SKOV-3 / half, DDP inhibitory concentration( IC50) were 38.486 ug / ml, 28.280 ug / ml, non-cytotoxic concentration(of IC5) was 3.212 ug / ml, 4.052 ug / ml.IC50 Curcuma oil injection on SKOV-3 and SKOV-3 / DDP inhibited the proliferation showed a time-dependent manner, a positive correlation between aging; aging Curcuma oil injection in the first 0-48 h time period than higher, so experiment Curcuma oil injection role as an observation node 48 h.Carboplatin SKOV-3 and SKOV-3 / DDP IC50 of 46.363 ug / ml, 186.003 ug / ml, resistance index(RF) of about 4.012, indicating carboplatin SKOV-3 / DDP resistance stability reliable.Proliferation IC5 Curcuma oil injection and IC50 carboplatin group and IC50 carboplatin positive control group, the inhibition rate higher than that in negative control group and IC5 Curcuma oil injection group, IC5 Curcuma oil injection group and blank negative control group no significant difference between, associated with cell proliferation inhibition rate was higher than the IC50 carboplatin positive control group(P <0.05).IC5 Curcuma oil injection of carboplatin resistant reversal index(RI) of about 2.166, indicating IC5 Curcuma oil injection can effectively reverse the SKOV-3 / DDP carboplatin resistance.Conclusion:Curcuma oil injection can effectively inhibit ovarian cancer sensitive cell line(SKOV-3) and cisplatin-resistant cell lines(SKOV-3 / DDP) in vitro proliferation and Curcuma oil injection on SKOV-3 and SKOV-3 proliferation / DDP showed some inhibitory concentration and time dependent manner.Curcuma oil injection can increase carboplatin SKOV-3 / DDP cytotoxic enhance carboplatin SKOV-3 / DDP proliferation inhibition, and enhance SKOV-3 / DDP sensitivity to carboplatin, reversal of SKOV-3 / DDP carboplatin resistance.