Study On The Reversal Effect Of Medroxyprogesterone Acetate On Cisplatin-resistant Ovarian Cancer Cell Lines SKOV3/DDP

Objective: To study the reversing effect of medroxyprogesterone acetate on SKOV-3/DDP cells in vitro, initially to explore its mechanism of resistance reversal.Methods: Using methylthi azolyl tetrazolime (MTT) assay to examine the cytotoxic effect of MPA to SKOV3/DDP cells and identify the nontoxic dose of MPA as reversed dose. Using the same assay to observe the changes of DDP resistance after cells were treated with nontoxic dose of MPA. The cells of cell cycle and apoptosis rate changes was monitored by flow cytometry (FCM). Results: When MPA's concentration was high than 30μg/ml could inhibitthe growth of SKOV3/DDP cells and the inhibition was concentration dependent. When MPA's concentration was low than 15.10μg/ml, MPA could not obviously proliferation inhibiting , and the experiment choose 15μg/ml for reversed dose. After being treated with DDP and 15μg/ml MPA combined for 24,48,72h,the IC50 values of decreased to 57.72±0.48μg/ml , 13.39±0.21μg/ml, 7.93±0.18μg/ml, the RF are 1.22,1.90 and 2.44. DDP combined MPA effect on the cells, the cell cycle arrest in G0/G1 phase ,declining proportion of S phase cells and increased the rate of resistance to apoptosis. Conclusion: MPA can reverse the resistance of SKOV3/DDP. The possible mechanism of MPA reversal of drug resistance may be increased the apoptotic rate of resistant cells,blocking the progression of the cell cycle.Conclusion1 MPA had anti-SKOV3/DDP Activity,and in a concentration dependent.2 MPA can reverse the resistance of SKOV3/DDP in cisplatin-resistant ovarian cancer cell line SKOV3/DDP. 3 MPA arrest cell cycle in the G0/G1 phase and increased the apoptotic rate of resistant cells.