The Role Of Sex Hormone In Uroseptic Shock:Animal Model Study

Objective:Through comparing different hormone levels in uroseptic shock animal model and the variation outcome of septic shock, we aim to explore the relationship between septic shock and sex hormone.Methods:30 male New Zealand rabbits were randomly assigned into control group A, experimental groups B and C,10 female New Zealand rabbits were included in experimental group D. Rabbits in Group B and C underwent surgical castration prior to the operation and rabbits in Group C were injected exogenous testosterone 24hours prior to the operation. During the operation, all rabbits in four groups were injected of Escherichia coli solution 2ml/kg into the renal pelvis to create the uroseptic shock model. Preoperative and postoperative mean arterial pressure, survival rates of all four groups were compared.Results:There was no significant difference between group A and group B,C in serum testosterone level before castration. Two weeks after castration, serum testosterone level was significant lower in group B and C compared to group A. In group C, there was no significant difference in serum testosterone level between pre-castration and injection of exogenous testosterone 24hours later.In the uroseptic shock model, MAP of all time sets in group B and D were significant higher than in group A; there was no significant difference between group C and A in MAP of all time sets, and MAP in group B of all time sets were significantly higher than group C. Compared to group A, shock arrived much later in group B and D, and there was no significant difference between group C and A regarding the time of shock's arrival. The results suggested that low level of androgen could postpone the process of uroseptic shock.8 hours post-operation death rate were as following:40%(4/10)in group A,0%(0/10)in group B,30%(3/10) in group C,0 (0/10) in group D; 10 hours post-operation death rate were as following:90%(9/10)in group A,10%(1/10)in group B,80%(8/10) in group C,0 (0/10) in group D; 12 hours post-operation death rate were as following:100%(10/10) in group A,60%(6/10) in group B,10%(10/10) in group C,50%(5/10) in group D. The results showed that low level of androgen could decrease mortality.Conclusion:In animal model experiment, we found that low level of androgen could postpone the process of uroseptic shock and decrease mortality. However, the role of estrogen in septic shock is still perplexing. Therefore, further research of estrogen's effect on septic shock is required.