| Objective: To observe the efficacy and safety of Momeiasone Furoate Cream and Wet sores aerosol combined utilization in patients with subacute eczema.Methods: All the subjects in this study came from the patients who were treated in the Dermatology clinic of The First Affiliated Hospital of Dalian Medical University within the time period lasting from Feb 2015 to Jan 2016.All patients satisfied the diagnostic criteria of subacute eczema and the requirement that major lesions distribute over the body less 10% of the total surface area.The patients were enrolled and randomly classified into two groups.The treatment group is treated with topical Momeiasone Furoate Cream combined with Wet sores aerosol.While the control group is treated with Wet sores aerosol alone.The Momeiasone Furoate cream is applied once a day,the Wet sores aerosol is applied forth a day.The courses of treatment lasted for 14 days.The response in patients before the treatment,7 days after the treatment and 14 days after the treatment was observed,respectively.Results: Totally,64 of the 70 outpatients with subacute eczema finished the test.,Of whom 35 were male and 29 were female,the treatment group were 34 subjects,the control group were 30 subjects.The average age was 45.7±19.45.After one week of treatment,the average SCORAD value in both groups decreased,but there existed no significant difference between the two groups l(P>0.05).After two weeks of treatment,the average SCORAD value decreased even further.The difference between the two groups was not statistically significant(P>0.05).After one week of treatment,the effective rate in treatment group was 79.4%,and the effective rate in control group was 70.0%,there was no significant difference between these two effective rates(P>0.05).After two-weeks of treatment,the effective rate in treatment group was 94.1%,and the effective rate in control group was 76.6%,there was significant difference between these two effective rates(P<0.05).No obvious adverse reaction appeared in the test.In the self-evaluation,most patients report that Wet sores aerosol has good attributes,and easy to use,and that their lesions and the quality of life all improved.In conclusion,the effective rate in patients treated with topical Mometasone Furoate Cream combined with Wet sores aerosol was significantly higher than that in patients treated with Wet sores aerosol alone.Conclusion: The combination with topical Wet sores aerosol may enhance the therapeutic effect of topical Momeiasone Furoate Cream on subacute eczema.Arsenic is a known environmental toxicant and a common drinking water contaminant.Chronic arsenic exposure results in various human skin lesions such as squamous cell carcinoma(SCC),basal cell carcinoma(BCC),and Merkel cell carcinoma(MCC).Ha Ca T cells are immortalized human keratinocyte cell line,no tumor characteristics.Studies shows that the Ha Ca T cells,when exposed chronically for >25 weeks to a low level of inorganic arsenic(100 n M)results in cell excessive abnormal growths,shape change,increase of cloned cells growth out of control,and cells able to produce aggressive SCC upon inoculation into nude mice.This Ha Ca T cells malignant phenotype(Arsenic-transformed cell,named AS-TM ??)can be used as a cell model to further study about the pathogenesis of skin cancer induced by arsenic.The micro RNA(mi RNA)is a form of small,single stranded RNA,18–25 nucleotides long.It is transcribed from DNA,instead of being translated into protein,and regulates the functions of other genes in protein synthesis.Therefore,mi RNAs are genes that modulate other protein coding genes.A growing number of studies show that mi RNAs plays a very important role in the development of tumor.However,the relationship between differentially expressed micro RNA with arsenic-induced Ha Ca T malignant transformation remains to be elucidated.ObjectiveTo investigate the differentially expressed micro RNA during arsenic-induced Ha Ca T malignant transformation and preliminary research of their function for in depth study of skin cancer induced by arsenic.Methods 1.The Ha Ca T cells were exposed chronically to a low level of arsenic,then analyzed by mi RNA microarray to find differentially expressed mi RNAs,and validated by real-time PCR.2.The AS-TM cell was transfected with RNA oligonucleotide respectively(mir-513a-5pm mimics,mir-181b-5p inhibitor or corresponding control RNA)using lipofectamin 2000 and the nontransfected cell was set as blank control group.We used real-time PCR to analyze the expression of 513a-5p and 181b-5p in different cell groups and evaluate the transfection effect.3.Cell proliferation assay(CCK8),Flow cytometry were used to measure the biological behavior changes in AS-TM cell after enhanced mir-513a-5p and inhibition of mir-181b-5p.Results1.Compared with the Ha Ca T cells,12 mi RNA genes were up-regulated and 14 down-regulated in the AS-TM cells.The differential expression of up-regulated mi RNAs: has-mir-6739-5p,has-mir-4521,has-mir-181b-5p,has-mir-100-5p,has-mir-3919,down-regulated mi RNA:has-mir-513a-5p,The results identified by microarray were the same as that by real-time PCR.2.mir-513a-5p mimics,mir-181b-5p inhibitor and both negative control RNA were transfected into the cell model successfully,q RT-PCR verified that mir-513a-5p expression levels increased significantly in cells model after transfected with mimics whereas mir-181b-5p was down-regulated after transfected with inhibitor.Remarkably,there was no distinct differences in blank group and negative control group.3.In cell proliferation and apoptosis assay indicated that there were no significant differences among all the gropes.Cell cycle analysis showed that inhibitor group increased the percentage of cells in S-phase and decrease G2-phase compared with the negative control group,the overexpression gro Up have no significant differences.Conclusions1.The expression of mi RNAs changes during arsenic-induced Ha Ca T malignant transformation,and mi RNAs may play a role in the formation,development and evolution of arsenic-induced skin diseases.2.The lower regulation of mir-181b-5p expression may effect the cell cycle of the AS-TM cells. |
PDF Full Text Request