Study On ELMO2 Regulating Colon Cancer Invasion And Metashsis

Objective:This study is designed to detect the differential expression of ELMO2 in human colon tissue and the healthy colon tissues;and to investigate the effects of ELMO1 on the biological behavior of colon cancer cells in vitro.Methods:1,Immunohistochemical staining was employed to detect positive expression rate of ELMO2 protein in 75 cases of colon cancer tissues.In order to seek the relationship between ELMO2 and clinicopathologic factor.We also analyzed the correlation between ELMO2 expression level and tumor pathological staging.2,qPCR was employed to detect expression of ELMO2 mRNA.Western blot was used to determine ELMO2 at the protein level in 75 cases of colon tissues and those corresponding colon cancer tissues.3,Wound-healing assay and transwell invasion assay were employed to detect migration and invasion of HCT116 colon cancer cell lines,before and after gene silencing using ELMO2 si RNA.Results:1,Immunohistochemical analysis showed that the positive expression rate of ELMO2 in colon cancer tissues was 54.67%.With the increase of tumor pathologic stage development,the expression of ELMO2 increases.2,qPCR and Western blot analysis showed that expression levels of ELMO2 m RNA and protein in colon cancer tissues were significantly higher than that seen in healthy colon tissues(P<0.01).3,Wound-healing assay showed that the closure of ELMO2 siRNA group was significantly slower than that of control group and invalid sequence group.Transwell invasion assay showed,after treatment with ELMO2 si RNA,the number of migrated HCT116 cells that passed through matrigel were significantly decreased as compared with the cells treated with control group and invalid sequenced control si RNA(P<0.01).Conclusion: The down-regulated expression of ELMO2 may significantly inhibit invasion and metastasis of colon cancer cell lines in vitro.