The Molecular Mechanisms Of Suppression Of Colon Cancer Invasion And Metastasis By Selective Cyclooxygenase-2 Inhibitor Nimesulide

Background and Objective: Cyclooxygenase-2(COX-2) is highly related with the incidence of colon cancer, and there is a high expression of COX-2 in colon cancer. Nonsteroidal anti-inflammatory drugs (NSAIDs) can decrease the incidence of colon adenomas and the risk of colorectal cancer, and the mechanism is mostly considered to be the inhibition of COX-2 activity. Nowadays the studies of NSAIDs on carcinoma are mainly concentrated on proliferation and apoptosis all over the world, while the study on invasion and metastasis is scarce. Aiming at the aspect of invasion and metastasis, we studied the influence of selective cyclooxygenase-2 inhibitor Nimesulide on the molecules related with invasion and metastasis in this study, experimenting on colonic cells Caco-2 which has a high expression of COX-2. This study is also to investigate the molecular mechanisms of suppression of colon cancer invasion and metastasis by selective cyclooxygenase-2 inhibitor.Methods: The human colon cancer cells Caco-2 were cultured. After treated with Nimesulide at various concentrations, the expression of E-cadherin was detected by Western Blotting, the expression of CD44v6 was detected by immunocytochemistry method, and the expression of MMP-9 mRNA was detected by real-time fluorescent quantitative RT-PCR.Results: Nimesulide increased the expression of E-cadherin in a dose-dependent way. And Nimesulide reduced the expression of CD44v6 in a dose-dependent way. Exposed to Nimesulide, the expression level of MMP-9 mRNA is decreased in a dose-dependent way.Conclusion: Selective cyclooxygenase-2 inhibitor Nimesulide can alter the expressions of the molecules related with invasion and metastasis (E-cadherin, CD44v6, MMP-9), reducing the ability of invasion and metastasis in colon cancer. This study preliminarily clarified the molecular mechanisms of suppression of colon cancer invasion and metastasis by selective cyclooxygenase-2 inhibitor.