Effects Of Tripterygium Wilfordii On Diabetic Mice Induced By STZ

BackgroundType 1 diabetes mellitus(T1DM), also known as autoimmune diabetes.formerly known as juvenile diabetes. This call has been seldom used, because in the past our understanding of diabetes is insufficient.. T1DM patients, accounting for about 10% of the total patients with diabetes mellitus. According to the 2015 International Diabetes Federation(IDF) released survey data, there are around 542 thousand children with type 1 diabetes. The main harm of diabetes is vascular and organ damage caused by chronic hyperglycemia, caused by chronic complications in patients with one or several diabetes. Such as the common cardiovascular disease, diabetic nephropathy, seriously affect the patient's quality of life and even eventually lead to death. Compared with the pathogenesis of type 2 diabetes, T1DM is very different, because it is the result of autoimmune diseases. The mechanism may be due to the destruction of the immune system of the insulin producing islet beta cells, the main feature of the lack of insulin secretion, so patients must inject insulin, and rely on life.The beta cell is an important component of islet cells, accounting for nearly 80% of islet cells. Some viruses(such as mumps virus, Coxsackie B4 virus and rubella virus) invasion of pancreatic islets were infected, some toxic chemicals(such as pyriminil, streptozotocin, four oxygen pyrimidine) will the damage of islet beta cells, resulting in its non immune injury, or due to the destruction of the beta cells, releasing some induced allergic protein causing autoimmune injury, including the participation of cellular immunity and humoral immunity and lead to islet autoimmune inflammatory injury and apoptosis and lead to serious damage to islet beta cells. In which streptozotocin(STZ) is a very mature animal model of diabetes construction reagents, the islet beta cells have a specific toxic effect of. Repeated doses of moderate doses of the method can cause a lot of damage to the rodent's beta cells for a long time, sustained islet inflammation, and the incidence of human T1DM is similar.The treatment of connective tissue diseases and autoimmune diseases. Tripterygium wilfordii Hook F also known as yellow rattan, is a traditional Chinese herbal medicine. Euonymus Corey public rattan plant of the genus with promoting blood circulation to remove blood stasis, heat clearing and detoxicating, detumescence node and other effects, also has anti-inflammatory, treatment of neural degenerative diseases and so on many kinds of pharmacological activity. In recent years in the field of diabetic nephropathy has been the use of the relevant clinical research experience in the treatment of. According to the domestic application of tripterygium glycosides treatment of diabetic nephropathy metaanalysis showed that tripterygium glycosides can reduce the concentration of albumin in urine in patients with diabetic nephropathy, and serum creatinine(SCR) and blood urea nitrogen(BUN) no obvious effect.Another reported in the literature, the other active ingredients:, you can activate the apoptotic protease Caspases, which induces apoptosis of peripheral T cells, while the thymus cells do not have an impact. Other studies have found that it can effectively activate the Caspase 3, and induce a dendritic cell(DC) apoptosis, which plays a role in antigen presentation, which can inhibit the cellular immune response. Effect on humoral immunity, Jianan Ren et al. Studies have shown that tripterygium glycosides can effectively make the serum C-reactive protein(CRP) and tumor necrosis factor(TNF-?) and interleukin-1?(IL-1?) significantly reduced. Yang Fan and others found that the preparations were able to reduce the inflammatory response by inhibiting the production of interleukin-6(IL-6) by macrophages.ObjectiveTo investigate the effects of Tripterygium wilfordii on the function of beta cells in type1 diabetes induced by STZ.1.To establish the experimental mouse model of type 1 diabetes mellitus8 week old healthy C57BL/6 mice were randomly divided into two groups after one week adaptive feeding. In addition to the control group, the STZ group and the STZ+Tripterygium wilfordii group were Injection of the STZ to model process.2. The STZ+Tripterygium wilfordii group gavage experimentsThe STZ+Tripterygium wilfordii group daily at 9 in the morning for Tripterygium gavage treatment, the remaining two group received saline for control experiments.3. Detection of mice feeding, weight and general health monitoring blood glucose and other indicators.Check the status of mice daily living environment, such as mental state, diet, exercise, shiny coat etc.. Weekly monitoring of blood glucose, body weight, diet etc.. At the end of the experiment, the mice were killed, blood and tissue materials were collected, properly fixed and frozen.4. Histological observationThe pancreatic tissues were paraffin sections after stained by hematoxylin- eosin, observed changes in islet cells under the microscope.5. Using ELISA to detect INS, IAA levelAfter blood centrifugation, the levels of insulin(INS) and insulin autoantibodies(IAA) in mouse plasma were detected.Results1. Compared with the control group, the body weight of STZ group was significantly reduced.2. After 6 weeks of treatment, the oral glucose tolerance test and the area under the blood glucose curve showed that the glucose tolerance was significantly improved in the STZ+Tripterygium wilfordii group compared with the STZ group.3. Compared with the control group, the INS group STZ was significantly reduced, the level of STZ+ Tripterygium wilfordii in INS group was higher than that in STZ group, while the level of IAA in STZ group was lower than that in STZ+ Tripterygium wilfordii group.4. HE staining display that the control group of islet cell density, the ordered gap is small, plump cytoplasm nucleolus. The STZ group of islet cell disorder shrinkage, vacuoles, nuclear dense, less cytoplasm. The STZ+Tripterygium group of islets although not triptolide shrinkage, but compared with islet cells arranged in disorder, there is sparse cell vacuoles.ConclusionTripterygium wilfordii on STZ induced type 1 diabetic mice islet cells has a protective effect, can reduce the mouse insulin autoantibodies, reduce the autoimmune reaction, so that the destruction of the beta cells eased, restoration of beta cell insulin secretion.