Effects Of Duxiao Ganqing Pill On The Expression Of IL-6?IL-8 Protein In Intestinal Tissue In Rats With Liver Cirrhosis

Objective:through the experiment to observe the toxic liver for clearing the elimination of liver cirrhosis rat intestinal pathological morphology and intestinal mucosa tissue of interleukin 6(IL-6),interleukin 8(IL-8)protein expression and the effect of explore toxic liver for clearing the elimination of cirrhosis of the liver damage mechanism of intestinal mucosal barrier function in rat.Methods:88 experiments with rats,to adapt to the environment raised seven days were randomly divided into 6 groups: normal group,model group,lactulose,toxic liver for clearing the high,medium and low dose group.Groups except normal group rats given 50% carbon tetrachloride solvent thighs subcutaneously,each only 0.5 ml,100 g-1,2 times,wk-1 a,and according to the weight to adjust dosage,drug use 12 weeks in a row,the preparation of cirrhosis of the liver model.After the success of the building,the normal group and model group rats given distilled water to fill the stomach,the rest of the group rats were filled with the corresponding drug.Normal group: under the same conditions in the same period,the daily given distilled water 2 ml / 100 g lavage;Model group: at the same time under the condition of the same breeding,daily given distilled water2 ml / 100 g lavage;Toxic liver elimination for clearing the high dose group(223.4 mg/kg): a lavage for medicine,for 12 consecutive weeks;Toxic dose group in liver for clearing the elimination(111.7 mg/kg): a lavage for medicine,for 12 consecutive weeks;Toxic liver elimination for clearing the low dose group(58.9 mg/kg): a lavage for medicine,for 12 consecutive weeks;Lactulose group(concentration of 100 g/L,with 1 ml / 100 g lavage): a lavage for medicine,for 12 weeks in a row.Continuous lavage for 12 weeks,all rats using 3% chloral hydrate anesthesia,open the abdominal cavity,the terminal ileum tissue 3-4cm,with 0.9% saline flushing,blot moisture,rapid snapping the ileum tissue after comparing observation is divided into two parts,put in 10% formalinsolution,respectively for intestinal histopathological examination and immunohistochemical examination.Results: 1,toxic liver for clearing the general state of cirrhosis rats:the effects of toxin elimination liver for clearing the each dose group and lactulose group compared with model group have different degrees of improvement,the elimination of liver poisoning for clearing the each group is better than that of lactulose group,and to improve the most obvious dose group,but there is still a gap in the normal group.Toxic liver for clearing the elimination of high,middle,low dose group,lactulose group and model group rats mortality rates were 43.75%,31.25%,37.5%,37.5%,37.5%,is the lowest dose group in liver for clearing the elimination.2,toxic liver for clearing the elimination of the influence of the liver cirrhosis rat intestinal tissue pathological morphology: score comparison,after scoring model group was obviously higher than that of normal group(P <0.05);Toxic liver for clearing the elimination was evidently improve each dose group,is better than that of lactulose group(P < 0.05 or P < 0.01),in which the optimal dose group,but the score is still higher than normal group(P <0.01);Lactulose group compared with model group,no significant difference(P >0.05).Illustrate toxic liver for clearing the high,medium and low dose group of intestinal tissue of liver cirrhosis rats were mitigation and protection.3,toxic liver for clearing the elimination of liver cirrhosis rat intestinal mucosa IL-6,IL 8 expression: the influence of model group rats intestinal mucosa IL-6,IL-8 expression is significantly higher than normal group(P? 0.01);Toxic liver elimination for clearing the high,medium and low dose group compared with model group rats intestinal mucosa IL-6,IL-8 expression decreased obviously(P ? 0.01),but still higher than normal group(P ?0.05);Toxic liver elimination for clearing the low dose group of rat intestinal mucosa IL-6,IL-8 expression,and lactulose group was no significant difference(P?0.05).Toxic liver for clearing the elimination of high,middledose group of rat intestinal mucosa IL-6,IL 8 expression below lactulose group(P ? 0.05,or P ? 0.01),of which the most obvious dose group.Illustrate toxic liver for clearing the liver cirrhosis rat intestinal mucosa the expression of IL-6,IL-8 has certain effect.Conclusion: 1,toxic liver for clearing the elimination can improve the general condition of liver cirrhosis in rats so as to improve the quality of life.2,toxic liver for clearing the elimination can improve liver cirrhosis rat intestinal tissue pathological morphology,and protect the integrity of the intestinal mucosa.3,toxic liver pills to a certain extent,can inhibit the intestinal mucosa tissue expression of IL-6,IL-8,reduce inflammation,regulating the barrier function of bowel mucosa,its toxic liver for clearing the elimination effect of high,middle dose group was obviously,and its overall effect is better than that of lactulose group;And low dose group and lactulose group is no significant difference.