Study On The Protective Effects Of Different-Dose Glutamine On The Intestinal Barrier In Young Rabbits With Hemorrhagic Shock

objectives: Through establishing the model of young rabbits with hemorrhagic shock, to study if Gln in different doses had protective effects on intestinal mucosal barrier and whether there would be intestinal bacterial translocation led to systemic inflammatory responsive syndrome(SIRS) which could be inhibited by Gln during hemorrhagic shock. Methods: 18 white young rabbits were randomly divided into three groups : Non-glutmine(N-Gln), Low-dose glutamine(L-Gln) and High-dose glutamine(H-Gln) groups. Three groups were fed normally ,while L-Gln and H-Gln glutamine groups were supplemented with glutamine at 0.5g/kg.day and 1.0g/kg.day by gastric tube respectively. All rabbits suffered hemorrhagic shock by blood withdrawing from femoral artery after 7 days feeding. Plasma levels of diamine oxidase(DAO) and serum levels of interleukin-8(IL-8) were measured at different time points including before shock, 2h ,6h and 24h after successful resuscitation of shock. Ileum tissue at 5 cm away from approximal ileocecal portion was removed and the change of morphology , distribution of lymphocyte , count of PMN and villous height, width and surface area were examined and measured. Results : Blood levels of DAO and IL-8, the amount of PMN and distribution of lymphocyte in intestine were decreased significantly in L-Gln and H-Gln groups compared with N-Gln group(p<0.01or p<0.05). Villous epithelial cells exfoliation, atrophy occurred and the height and surface area were declined in N-Gln group compare with L-Gln and H-Gln groups (p<0.01 or p<0.05), but no difference of villous width in three groups. There were no difference in all parameters measured and tissue morphology between L-Gln and H-Gln groups(p>0.05) . Conclusions: In available range, glutamine has potential effects on intestinal mucosal barrier protection and on SIRS process inhibition.