Studies On The Chemical Components, Biological Activity Of Hemsleya Jinfushanensis

Hemsleya amabilis belongs to Cucurbitaceae Hemsleya Cogn, and they are distributed throughout Asia subtropical to temperate regions. The main chemical constituents of Hemsleya are triterpenoid saponins. The tubers of Hemsleya amabilis are "bitter drugs with cooling properties, dissipating poisons, and repelling swellings", and they are used as antibacterial remedy to treat bacillary dysentery, enteritis, bronchitis and acute tonsillitis, etc.Objective: 1) To study the chemical constituents of Hemsleya amabilis. 2) To study the bioactivity of all compounds on different cancer cells. 3) To explore the mechanism of anti tumor activity by the experiment of angiogenesis inhibition in zebrafish.Methods: The ethyl acetate extract and n-butanol part were isolated and purified by means of chromatography(silica gel column, Sephadex LH-20 column, ODS column, pre-HPLC) and re-crystallization. Their structures were determined on the basis of ultraviolet spectrum, infrared spectrum, mass spectrometry and nuclear magnetic resonance spectroscopy technology; The cytotoxic activity of 11 compounds against H460 cells(human lung adenocarcinoma) ?DU145(human prostate cancer) and SW-620(human colon cancer) were evaluated by MTT method; Use the transgenic zebrafish(VEGFR2: GFP) as the experimental animal model, observe the effect of different doses on intersegment vessels(ISV) under the fluorescence microscope.Results: 26 compounds were isolated from the H. jinfushanensis. Their structures are identified as: Hemslepencins A(1), Hemslepencins B(2), Hemslepencins C(3), Hemslepencins D(4), 16,25-Odiacetyl-cucurbitane F(5), Hemslepenside B(6), Hemslepenside C(7), Hemslepenside D(8), Hemslepenside E(9), Hemslepenside F(10), Hemslepenside G(11), Hemslepenside H(12), Hemslepenside I(13), 3?-hydroxycucurbita-5,24-diene-11-one-26-methy-27-O-?-D-glucopyranosyl(1?6)-b-D-gluc opyranoside(14), 3b,11,26-trihydroxycucurbita-5,24-dien-26-O-b-D-glucopyranosyl(1?6)-b-D-glucopyranoside(15), Hemslecins G(16), Hexanorcucurbitacin F(17), Scandenogenin A(18), 23,24-dihydrocucurbitane F-16,25-diacetate(19),25-Oacetyl-23,24-dihydro-cucurbitane F-2-O-b-D-glucopyranoside(20), jinfushanosides B(21), jinfushanosides G(22), delavanosides D(23), Carnosifloside I(24), jinfushanosides C(25), jinfushanosides D(26)? Some compounds were choose to test cytotoxic activity, and the results showed that the new compounds 5, 6, 7 and 8 had strong cytotoxicity. All compounds are test for the angiogenesis inhibition in zebrafish, compounds 2, 3, 5, 6, 8, 10, 19 have certain inhibitory effect.Conclusion: 26 compounds were isolated from H.jinfushanensis, including 13 new compounds, 1 compound first isolated from nature, 12 known compounds. Compound 5, 6, 7, 8 had strong cytotoxic activity, and compound 2, 3, 5, 6, 8, 10, 19 have certain inhibitory effect.