The Differential Expression Of Long Non-coding RNAs In Osteogenesis Imperfect And The Epidemiological Analysis Of OI Patients(?) The Epidemiological Analysis Of OI Patients(?)

Long noncoding RNA(long non coding RNA, LncRNA) is a kind of RNA transcription of this length 200 ~ 200 nucleotides, which have no coding protein ability or rarely have the ability of encoding proteins. LncRNA through epigenetic regulation and transcription regulation and transcription regulation to regulate the expression of related target genes.As a growing number of LncRNA was found in the human genome, lnc RNA formwith the nervous system and cardiovascular form and cancer occurrence and development is the relationship between was reported. And its expression level stage of development is closely associated with disease. Osteogenesis insufficiency(osteogenesis imperfecta, OI) is a type of ? collagen synthesis and metabolic disorders caused by a hereditary connective tissue diseases.Main clinical manifestation is easy to fracture, osteoporosis, some patients with blue sclera, and dentin dysplasia, hearing loss, muscle weakness, the symptom such as joint ligament relaxation. This study intends to analyze 14 kinds of long chain non-coding RNA in patients with osteogenesis differentially expressed in bone tissue, lay a foundation for the study of bone lncRNA and its related functions.Objective: To explore the differential expression of 14 kinds of long non-coding RNAs in bone tissues derived from osteogenesis imperfecta patients.Methods: RT-qPCR was performed to detect the expression level of 14 Kinds of lncRNAs including ATB, EBIC, HEIH, hLACR1, HOTAIR, PVT1, LET, Loc285194, SRHC, LSINCTS, Nbla10727, Nbla12061, PRNCR1 and UC.388 in bone tissues derive from osteogenesis imperfect(OI) patients who received corrective surgery. Bone tissues from developmental dysplasia of the hip(DDH) were used as control. REST-2009 was performed to analyze the results.Results: The expression of PRNCR1 was significantly down-regulated in OI bone tissues. There's no differential expression was observed in other thirteen lncRNAs, though the expression of HEIH, hLACR1, Nbla10727, Nbla12061 and UC.388 were up-regulated and LSINCTS was down-regulated. The expression of ATB,EBIC, HOTAIR, PVT1, LET, Loc285194 and SRHC was less than 2-fold of DDH control.Conclusions: Low expression of PRNCR1 was observed in bone tissue from OI patients. The functions of PRNCR1 in OI need further study.