Purpose:This research we performed aimed to screen preliminary differentialexpression bone-related microRNAs(miRNAs) in serum of patients with osteogenesisimperfecta(OI), and lay the foundation for further study about the diagnosisapplication of miRNAs in OI.Methods: Firstly, we devoted ourselves to select the reference gene(s) fit forquantitative detection of serum miRNAs by using geNorm and several otherprogrammes. Then real-time fluorescent quntitative PCR was used to detect theexpression level of bone-related miRNAs gained by means of miRanda,Targetscanand Pictar softwares caculation and reading literature. Finally, the results wereanalyzed with the matched t test.Resμlts: All6candidate reference genes had a stable expression level in serum ofhealthy controls and patients with different characters, and the optimal number ofreference genes is4(miR-16, let-7a, snRNAU6, miR-92a) after Pairwise Variationsanalysis (V4/5=0.133<0.15). For validating the universality of expression stability, wedetected the relative expression value of miR-16, let-7a, snRNAU6and miR-92a inanother8healthy controls and16patients with OI and the result revealed that theexpression of4genes remained stable (M<1.5). After measuring serum levels of morethan100bone-related miRNAs in patients with real-time qPCR,11miRNAs showed differential expression, and bioinformatic analysis suggested these alteredexpressional mioRNAs had possibilities to participate in the process of OI.Conclusion: There existed many differential expression bone-related miRNAs inserum of patients with OI, and these miRNAs had potentials to be promisingbiomarkers for serologic tests and diagnosis of OI. |