The Expression And Clinical Significance Of IGF-1 And IGFBP-3 In Glioma

Background:Glioma is the most common malignant tumor in central nervous system.There is a strong invasive in glioma, and there is no clear boundaries between normal tissue and glioma tissues, it is difficult to complete resection in the surgical operation, the glioma patients had a higher recurrence rate after operation,, the survival rate is low, whichis serious influence on people’s life quality. Insulin-like growth factor (IGF) participates in vertebrate cells proliferation and differentiation, growth, tissue repair peptide, there are some insulin-like growth factor binding protein (IGFBPs) in circulation system, IGF as a carrier, which has decisive effect on bioavailability of IGFs, insulin-like growth factor binding protein 3 (IGFBP-3) accounts for a major role. Research had showed that there were important relation between high level of insulin-like growth factor 1 (IGF-1) in serum and malignant tumor, malignant tumor patients had much higher expression.The expression level of IGF-1 increased significantly in glioma tissues, its expression was positively correlated with glioma malignant degree, as a powerful mitogen, IGF-1 could be applied directly to tumor cells, induced the split proliferation. IGFBP-3 could combine and transport IGF-1, and inhibit cell signaling pathways, regulate extracellular signal conditioning kinases and cell growth, promote apoptosis, and inhibit of glioma cell proliferation, as a tumor suppressor genes, IGFBP-3 might play a role. This objective of this study is to detect the preoperative and postoperative of peripheral the blood content and change of IGFBP-3 and IGF-1 in patients with gliomas, and to detect the proteins expression of local situation glioma tissue, to discuss the change in the occurrence and development of glioma.Objective:To explore the exact roles of IGF-1 and IGFBP-3 proteins in the development of glioma and to analyze their clinical significance in auxiliary diagnosis, risk prediction and therapy.Methods:① The contents and changes of IGF-land IGFBP-3 in serum of glioma group and control group pre and post operation were detected by chemiluminescence. ②mmunohistochemistry was used to detect the expressions of IGF-land IGFBP-3 levels in glioma tissues and normal brain tissues. ③Western blot was used for detecting the expressions of IGF-land IGFBP-3 levels in glioma tissues and normal brain tissues.Results:Compared to pre operation, the post operation contents of IGF-land IGFBP-3 in glioma group significantly decreased (P<0.01), while the data of control group have no obvious difference (P>0.05). No matter pre or post operation, the expressions of IGF-land IGFBP-3 in serum were irrelevant to the pathologic gradation of glioma (P>0.05). The results of immunohistochemistry and Western blot showed that the expressions of IGF-land IGFBP-3 significantly increased and were positively correlated with the pathologic gradation (rs=0.456, rs=0.479, P<0.05).Conclusions:IGF-land IGFBP-3 might promote the development of glioma and detect the contents and their change have important value on the diagnosis and prognosis.